GAPDH Antibody

Code CSB-PA00025A0Rb
Size US$166
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: GAPDH antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA00025A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA00025A0Rb at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA00025A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00025A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00025A0Rb at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GAPDH Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
38 kDa BFA-dependent ADP-ribosylation substrate antibody; aging associated gene 9 protein antibody; Aging-associated gene 9 protein antibody; BARS-38 antibody; cb609 antibody; EC 1.2.1.12 antibody; Epididymis secretory sperm binding protein Li 162eP antibody; G3P_HUMAN antibody; G3PD antibody; G3PDH antibody; GAPD antibody; GAPDH antibody; Glyceraldehyde 3 phosphate dehydrogenase antibody; glyceraldehyde 3-PDH antibody; Glyceraldehyde-3-phosphate dehydrogenase antibody; HEL-S-162eP antibody; KNC-NDS6 antibody; MGC102544 antibody; MGC102546 antibody; MGC103190 antibody; MGC103191 antibody; MGC105239 antibody; MGC127711 antibody; MGC88685 antibody; OCAS; p38 component antibody; OCT1 coactivator in S phase; 38-KD component antibody; peptidyl cysteine S nitrosylase GAPDH antibody; Peptidyl-cysteine S-nitrosylase GAPDH antibody; wu:fb33a10 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-335AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GAPDH Antibody (Product code: CSB-PA00025A0Rb) is Non-conjugated. For GAPDH Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA00025B0Rb GAPDH Antibody, HRP conjugated ELISA
FITC CSB-PA00025C0Rb GAPDH Antibody, FITC conjugated
Biotin CSB-PA00025D0Rb GAPDH Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
GAPDH polyclonal antibody CSB-PA00025A0Rb was produced in the rabbit immunized by using the Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-335AA) as the immunogen. The target protein GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
This Rabbit anti-Homo sapiens (Human) GAPDH Polyclonal antibody was tested in the ELISA, WB, IHC and IF. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the GAPDH proteins of human-origin and may be used to detect the endogenous levels of GAPDH protein.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 13 reviews

Submit a Review here

Applications : Western Blot (WB)

Sample type: hPSCs

Sample dilution: 1:10,000

Review: Expression and phosphorylation analysis of PGRMC1, GSK-3β, β-catenin, and Wnt3a in control or PGRMC1 knockdown hPSCs. Cell lysates were analyzed by Western blot analysis with indicated antibodies. GAPDH was used as an internal protein control and loading control.

By Anonymous

Applications : Western blot assays

Sample type: Human cells

Review: p-ATF4(S219) in SGC7901 (left) or BGC823 (right) with Flag-CK1δ over-expression was analysed by western blotting.

By Anonymous

Applications : Western blot assays

Sample type: Human cells

Review: The effect of Mg2+ on the protein levels of CDKL3 in the wild-type and the mutant HeLa cells. The protein expression of CDKL3 was quantified by band intensity of the western blots with normalization to GAPDH protein. The quantified band intensity is displayed as the relative amount to the proteins in wild-type HeLa cells incubated at 0 mM Mg2+.

By Anonymous

Applications : WB

Sample type: Human cells

By Anonymous

Applications : WB

Sample type: Human cells

By Anonymous

Applications : WB

Sample type: Human cells

By Anonymous

Applications : WB

Review: ADRP expression level from (k) was determined using the ImageJ software and was normalized using the GAPDH expression level; **p<0.01.

By Anonymous

Applications : Western Blot

Sample type: Muscle Myo-lineage cells

Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.

By Anonymous

Applications : WB

Sample type: Human Cell

Sample dilution: 1:5000

By Anonymous

Applications : WB

Sample type: Human Cell

Review: Western bolt assays showed that the protein levels of GAPDH immunoblots from control and respective knockdown cells.

By Anonymous

Applications : Western blots

Sample type: cells

Review: AREG protein levels in cytoplasmic and nuclear fractions of cells treated with BPTES, DMKG or both. GAPDH and LaminB1 were used as cytoplasmic or nuclear loading controls, respectively.

By Anonymous

Applications : Western blot analysis

Sample type: cell

Review: Western blot analysis was performed. Relative expression was calculated using GAPDH as a reference gene.

By Anonymous

Applications : Western Blot

Sample type: cells

Review: GAPDH and Lamin B1 were served as cytosolic and nuclear loading controls, respectively.

By Anonymous

 Q&A
Q:

I am very much interested to try out the antibody (CSB-PA00025A0Rb) since the brand I'm using now is proven to be non effective for my cell line.

A:

GAPDH Antibody (CSB-PA00025A0Rb) has been tested in ELISA, WB, IHC, IF succesfully. And we offer 100% risk-free guarantee for all antibodies, a refund or replacement will be provided if the antibody does not work for the applications/species stated on our website and datasheet.

Target Background

Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Gene References into Functions
  1. This suggests that RX624 might be useful as a drug against polyglutamine pathologies, and that is could be administered exogenously without affecting target cell physiology. This protective effect was validated by the similar effect of an anti-GAPDH specific antibody. PMID: 28450110
  2. GAPDH can interact with proteins participating in DNA repair, such as APE1, PARP1, HMGB1, and HMGB2. In this review, the functions of GAPDH associated with DNA repair are discussed in detail. PMID: 28601074
  3. Nitric oxide-induced GAPDH aggregation specifically induces mitochondrial dysfunction via permeability transition pore opening, leading to cell death. PMID: 28167533
  4. GAPDH may act as a chaperone in heme transfer to downstream areas PMID: 28315300
  5. NAD(+) inhibited both GAPDH aggregation and co-aggregation with GOSPEL, a hitherto undescribed effect of the coenzyme against the consequences of oxidative stress. PMID: 27282776
  6. Monoclonal Antibodies DSHB-hGAPDH-2G7 and DSHB-hGAPDH-4B7 Against Human Glyceraldehyde-3-Phosphate Dehydrogenase. PMID: 27556912
  7. the present study suggests that GAPDH plays an important role in cancer metastasis by affecting EMT through regulation of Sp1-mediated SNAIL expression. PMID: 27878251
  8. Knockdown of LAMP2A, a CMA-related protein, and TSG101, an mA-related protein, significantly but only partially decreased the punctate accumulation of GAPDH-HT in AD293 cells and primary cultured rat cortical neurons. PMID: 27377049
  9. In conclusion, the data show that two GAPDH binders could be therapeutically relevant in the treatment of injuries stemming from hard oxidative stress. PMID: 26748070
  10. transient silencing of GAPDH reduces intracellular ROS and facilitates increased autophagy, thereby reducing acute hypoxia and reoxygenation injury as well as the resulting apoptosis and necrosis. PMID: 26279122
  11. This review will summarize our current understanding of GAPDH-mediated regulation of RNA function PMID: 26564736
  12. analysis of PSCA level in the peripheral blood of PC patients who underwent radical prostatectomy shows it is related to a GADPH reference level (PSCA/GAPDH ratio) PMID: 26527100
  13. In 60 % of patients with type 2 diabetes, a reversible inhibition of GAPDH is observed. PMID: 25189828
  14. The results of this study led us to conclude that in cancer cells constantly exposed to conditions of oxidative stress, the protective power of Hsp70 should be abolished by specific inhibitors of Hsp70 expression. PMID: 26713364
  15. GAPDH and protoporphyrinogen oxidase were shown to have higher expression in faster growing cell lines and primary tumors. Pharmacologic inhibition of GAPDH or PPOX reduced the growth of colon cancer cells in vitro PMID: 25944804
  16. The levels of GAPDH protein were significantly up-regulated in lung squamous cell carcinoma tissues and elevated GAPDH expression is associated with the proliferation and invasion of lung and esophageal squamous cell carcinomas. PMID: 25944651
  17. Genetic variants in GAPDH confer susceptibility to sporadic Parkinson's disease in a Chinese Han population. PMID: 26258539
  18. Data revealed that GAPDH is a phosphorylation substrate for AMPK and its interaction with Sirt1 in the nucleus. The phosphorylation and the nuclear translocation of GAPDH mediate rapid Sirt1 activation and autophagy initiation under glucose deprivation. PMID: 26626483
  19. findings demonstrate that dissociation of the GAPDH/Siah1 pro-apoptotic complex can block high glucose-induced pericyte apoptosis, widely considered a hallmark feature of diabetic retinopathy PMID: 26438826
  20. Extracellular GAPDH, or its N-terminal domain, inhibited gastric cancer cell growth. GAPDH bound to E-cadherin and downregulated the mTOR-p70S6 kinase pathway. PMID: 25785838
  21. suggests that GAPDH aggregates accelerate Abeta amyloidogenesis, subsequently leading to mitochondrial dysfunction and neuronal cell death in the pathogenesis of AD PMID: 26359500
  22. The level of GAPDH-AP DNA adduct formation depends on oxidation of the protein SH-groups; disulfide bond reduction in GAPDH leads to the loss of its ability to form the adducts with AP DNA PMID: 26203648
  23. The activity of GAPDS was significantly positively correlated with sperm motility and negatively with the incidence of infertility. PMID: 26255202
  24. The N terminus of nuclear GAPDH binds with PARP-1, and this complex promotes PARP-1 overactivation both in vitro and in vivo. PMID: 25882840
  25. deregulated GAPDH expression promotes NF-kappaB-dependent induction of HIF-1alpha and has a key role in lymphoma vascularization and aggressiveness PMID: 25394713
  26. analysis of how flux through GAPDH is a limiting step in aerobic glycolysis PMID: 25009227
  27. astrocytic production of D-serine is modulated by glycolytic activity via interactions between GAPDH and SRR. PMID: 25870284
  28. Dimer and tetramer interface residues in adenine-uridine rich elements are important for GAPDH-RNA binding. PMID: 25451934
  29. Siah1 is a substrate of ASK1 for activation of the GAPDH-Siah1 oxidative stress signaling cascade. PMID: 25391652
  30. GAPDH expression is deregulated during melanoma progression. PMID: 25550585
  31. Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase. PMID: 25086035
  32. MZF-1 binds to and positively regulates the GAPDH promoter, indicating a role for GAPDH in calcitriol-mediated signaling. PMID: 25065746
  33. The protein encoded by this gene contains a peptide that displays antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. PMID: 22832495
  34. GAPDH gene over expression in resected tumor samples is an adverse prognostic factor in non small cell lung cancer. PMID: 23988223
  35. This review describes the structure and localization of GAPDH in cells as well as the latest discoveries on the multifunctional properties of the enzyme. PMID: 24018444
  36. TG2-dependent GAPDH deamidation was suggested to participate in actin cytoskeletal remodeling. PMID: 24375405
  37. acetylation of GAPDH (K254) is reversibly regulated by the acetyltransferase PCAF and the deacetylase HDAC5. PMID: 24362262
  38. GAPDH binds to active Akt, leading to Bcl-xL increase and escape from caspase-independent cell death. PMID: 23645209
  39. GAPDH is a moonlighting protein that functions as a glycolytic enzyme as well as a uracil DNA glycosylase. PMID: 20727968
  40. Results indicate that CIB1 is uniquely positioned to regulate PI3K/AKT and MEK/ERK signaling and that simultaneous disruption of these pathways synergistically induces a nuclear GAPDH-dependent cell death. PMID: 22964641
  41. The data presented demonstrate that up-regulation of GAPDH positively associated genes is proportional to the malignant stage of various tumors and is associated with an unfavourable prognosis. PMID: 23620736
  42. In a yeast two-hybrid screen of a heart cDNA library with Mst1 as bait, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as an Mst1-interacting protein. PMID: 23527007
  43. interaction between prolyl oligopeptidase and glyceraldehyde-3-phosphate dehydrogenase is required for cytosine arabinoside-induced glyceraldehyde-3-phosphate dehydrogenase nuclear translocation and cell death PMID: 23348613
  44. NleB, a bacterial glycosyltransferase, targets GAPDH function to inhibit NF-kappaB activation. PMID: 23332158
  45. GAPDH binds to alkylated, single-stranded, double-stranded and telomeric sequences in a drug-dependent and DNA sequence/structure-dependent manner. PMID: 23409959
  46. GAPDH negatively regulates HIV-1 infection and provide insights into a novel function of GAPDH in the HIV-1 life cycle and a new host defense mechanism against HIV-1 infection. PMID: 23237566
  47. The strength, selectivity, reversibility, and redox sensitivity of heme binding to GAPDH are consistent with it performing heme sensing or heme chaperone-like functions in cells. PMID: 22957700
  48. The ability of C1q to sense both human and bacterial GAPDHs sheds new insights on the role of this important defense collagen molecule in modulating the immune response. PMID: 23086952
  49. SIRT1 functions to retain GAPDH in the cytosol, protecting the enzyme from nuclear translocation via interaction with these two proteins. PMID: 22789853
  50. The present mini review summarizes recent findings relating to the extraglycolytic functions of GAPDH and highlights the significant role this enzyme plays in regulating both cell survival and apoptotic death--{REVIEW} PMID: 21895736

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Subcellular Location
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Database Links

HGNC: 4141

OMIM: 138400

KEGG: hsa:2597

STRING: 9606.ENSP00000229239

UniGene: Hs.544577

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