abd-A Antibody

Code CSB-PA333458XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) abd-A Polyclonal antibody
Uniprot No.
Target Names
abd-A
Alternative Names
abd-A antibody; CG10325Homeobox protein abdominal-A antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) abd-A protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for segmental identity of the second through eighth abdominal segments. Once a pattern of abd-A expression is turned on in a given parasegment, it remains on the more posterior parasegment, so that the complex pattern of expression is built up in the successive parasegments. Appears to repress expression of Ubx whenever they appear in the same cell, but abd-A is repressed by Abd-B only in the eight and ninth abdominal segments.
Gene References into Functions
  1. We also observed that these functions of abd-A are required in its exclusive as well as the coexpression domain with that of Abd-B PMID: 25340649
  2. This "iab-8 RNA" produces a micro RNA to repress abd-A, but also has a second, redundant repression mechanism that acts only "in cis." Transcriptional interference with the abd-A promoter is the most likely mechanism PMID: 22654672
  3. Abdominal-A uses both Pax2-dependent and Pax2-independent mechanisms to stimulate rhomboid Cis-regulatory-modules activity to induce proper oenocyte numbers. PMID: 22438572
  4. The results uncover redundancy, interactivity, and multifunctionality of protein domains as salient features underlying overall AbdA protein activity PMID: 22046139
  5. Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen. PMID: 20875816
  6. in order to make an oenocyte, abdA regulates just one principal target, rho, that acts at the top of a complex hierarchy of cell-differentiation genes PMID: 12050142
  7. abd-A expression in the dorsal vessel was detected only in the heart, and overexpression of abd-A induced heart fate in the aorta in a cell-autonomous manner. PMID: 12397110
  8. In the abdomen, grainy head CG5058-PA terminates neural proliferation by regulating the competence of neuroblasts to undergo apoptosis in response to Abdominal-A expression PMID: 16049114
  9. The relationship between Abd-B and abd-A in the female genital disc is opposite to that of the embryonic epidermis, and contravenes the rule that posteriorly expressed Hox genes downregulate more anterior ones. PMID: 16319117

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Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Database Links

KEGG: dme:Dmel_CG10325

STRING: 7227.FBpp0082829

UniGene: Dm.3286

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