ackA Antibody

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Code	CSB-PA358803XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ackA Polyclonal antibody

Uniprot No.

P0A6A3

Target Names

ackA

Alternative Names

ackA antibody; ack antibody; b2296 antibody; JW2293Acetate kinase antibody; EC 2.7.2.1 antibody; Acetokinase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ackA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. The main pathway for acetate production during exponential phase.

Gene References into Functions

Acetate and glucose fluxes in Escherichia coli are determined by the thermodynamic control of the Pta-AckA pathway. PMID: 28186174
pta/ackA recF mutants are viable, suggesting, together with the inviability of pta/ackA recBC mutants, that chromosomal lesions due to the pta/ackA defect are of the double-strand-break type. PMID: 15687190
Deletion of the E. coli ackA gene (together with deletions of other ferementation disruption genes) increased production of succinate PMID: 18781696

Subcellular Location

Cytoplasm.

Protein Families

Acetokinase family

Database Links

KEGG: ecj:JW2293

STRING: 316385.ECDH10B_2458

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ackA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background