AMT1-3 Antibody

Code CSB-PA879805XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AMT1-3 Polyclonal antibody
Uniprot No.
Target Names
AMT1-3
Alternative Names
AMT1-3 antibody; At3g24300 antibody; K7M2.7Ammonium transporter 1 member 3 antibody; AtAMT1;3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AMT1-3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ammonium transporter probably involved in ammonium uptake from the soil. Contributes with AMT1-1 to the overall ammonium uptake capacity in roots under nitrogen-deficiency conditions.
Gene References into Functions
  1. AMT1;1 and AMT1;3 form homo- and hetero-oligomers at the plasma membrane in plants. PMID: 23463773
  2. Results show that the clustering and endocytosis of AMT1;3 indicating an effective mechanism by which plant cells can avoid accumulation of toxic levels of ammonium by eliminating active AMT1;3 from the plasma membrane. PMID: 23882074
  3. These results show that ammonium is complementary to nitrate in shaping lateral root development and that stimulation of lateral root branching by ammonium occurs in an AMT1;3-dependent manner. PMID: 21119058
  4. Thus, AMT1 phosphorylation appears to function in a feedback loop restricting ammonium uptake. T PMID: 19948793
  5. The results of this study suggest an additive contribution of AMT1;1 and AMT1;3 to the overall ammonium uptake capacity in Arabidopsis roots under nitrogen-deficiency conditions. PMID: 17026539

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Ammonia transporter channel (TC 1.A.11.2) family
Tissue Specificity
Highly expressed in roots. Expressed in root tips, root hairs, root epidermis, rhizodermis and cortex.
Database Links

KEGG: ath:AT3G24300

STRING: 3702.AT3G24300.1

UniGene: At.5885

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