arfB Antibody

Datasheet

Code	CSB-PA334759XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) arfB Polyclonal antibody

Uniprot No.

P40711

Target Names

arfB

Alternative Names

arfB antibody; yaeJ antibody; b0191 antibody; JW0187Peptidyl-tRNA hydrolase ArfB antibody; PTH antibody; EC 3.1.1.29 antibody; Alternative ribosome-rescue factor B antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) arfB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Rescues stalled ribosomes. Can hydrolyze peptidyl-tRNA on ribosomes stalled by both non-stop mRNAs and mRNAs that contain rare codon clusters or ribosomes stalled in the middle of mRNA. First identified as a complementary ribosome rescue system when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system or the alternative ribosome-rescue factor A (arfA).

Gene References into Functions

the efficiency of the ArfA-dependent process decreases rapidly with an increase in mRNA length downstream of the A site of the ribosome whereas YaeJ function is maintained on mRNA with sufficient length. PMID: 22922063
3.2 A crystal structure of YaeJ bound to 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA; structure reveals the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes PMID: 22422986
In this study, the authors identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant. PMID: 21418110
Results suggest that YaeJ functions as a ribosome-attached rescue device for stalled ribosomes. PMID: 21051357

Subcellular Location

Cytoplasm.

Protein Families

Prokaryotic/mitochondrial release factor family

Database Links

KEGG: ecj:JW0187

STRING: 316385.ECDH10B_0172

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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arfB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background