Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF8 Polyclonal antibody

Uniprot No.

Q9FGV1

Target Names

ARF8

Alternative Names

ARF8 antibody; FWF antibody; At5g37020 antibody; K15O15.5Auxin response factor 8 antibody; Protein FRUIT WITHOUT FERTILIZATION antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ARF8 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development.

Gene References into Functions

these data argue against the previously reported role of increased ARF8 expression in developmental defects caused by P1/HC-Pro. PMID: 27688620
Analyses pinpointed misregulation of ARF8 as a major cause for the developmental aberrations exhibited by VSR transgenic plants, but also for the phenotypes induced during normal viral infection caused by the HcPro-encoding Turnip mosaic virus. PMID: 21589905
ARF8 influences cell expansion and proliferation during petal growth. PMID: 21421811
We concluded that ARF6 and ARF8, in parallel with AS1 and AS2, repress the class 1 KNOX genes in developing floral organs to allow progression of the development of these organs. PMID: 20007966
Phenotypic plasticity of adventitious rooting is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and microRNA abundance. ARF6 and ARF8, targets of miR167, are positive regulators. PMID: 19820192
We characterized the function of auxin response factor 8 in Arabidopsis by investigating a T-DNA insertion line (arf8-1) and overexpression lines (ARF8 OX) of ARF8. PMID: 15469491
Auxin response factors ARF6 and ARF8 promote jasmonic acid production, while their gene dosage affects the timing of flower maturation. PMID: 16107481
Enhance expression of microRNA, miR167, which has a complementary sequence to a portion of the AUXIN RESPONSE FACTOR 8 (ARF8), can cause plant fertility defect. PMID: 16699541
ARF8 restricts signal transduction processes in ovules and growth in pistils until the fruit initiation cue. PMID: 16829592
Data show that Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. PMID: 17021043
The results of this study suggest that similar mechanisms involving auxin signaling exist to inhibit parthenocarpic fruit set in tomato and Arabidopsis. PMID: 17766399

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Subcellular Location

Nucleus.

Protein Families

ARF family

Tissue Specificity

Expressed in the whole plant.

Database Links

KEGG: ath:AT5G37020

STRING: 3702.AT5G37020.1

UniGene: At.23446

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ARF8 Antibody

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