Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) ash1 Polyclonal antibody

Uniprot No.

Q9VW15

Target Names

ash1

Alternative Names

ash1 antibody; KMT2H antibody; CG8887 antibody; Histone-lysine N-methyltransferase ash1 antibody; EC 2.1.1.354 antibody; Absent small and homeotic disks protein 1 antibody; Lysine N-methyltransferase 2H antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) ash1 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. TrxG proteins are generally required to maintain the transcriptionally active state of homeotic genes throughout development. Does not act as a coactivator required for transcriptional activation, but specifically prevents inappropriate Polycomb Group (PcG) silencing of homeotic genes in cells in which they must stay transcriptionally active.

Gene References into Functions

Mrg15 is a subunit of the Ash1 complex, a stimulator of Ash1 enzymatic activity and a critical regulator of the TrxG protein function of Ash1 in Drosophila. PMID: 29158494
Ash1 HMTase activity is activated by MRG15, a subunit of the identified Ash1 complex, and that this complex, rather than the Ash1 protein alone, is the active form of this H3K36 methyltransferase, both in vitro and in vivo. PMID: 29540501
If ASH1 or TRX function is lost or reduced, heterochromatin can spread into these domains creating a sink that diverts heterochromatic proteins from other variegating locations, which then may express a suppressed phenotype. PMID: 27373142
Physical interaction of ASH1 with FSH activates global transcription. PMID: 23442797
loss of ASH1 significantly decreases H3K36 dimethylation, providing further evidence that ASH1 is an H3K36 dimethylase in vivo. PMID: 24004944
implication that ASH1 is required for essentially all H3-K4 methylation that occurs in vivo PMID: 13679578
lysine to arginine substitution of histone H3 at position 36 abolished the methyltransferase activity of Drosophila ASH1, suggesting that K36 is their specific target PMID: 17544230

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily

Tissue Specificity

Expressed throughout development but is present at higher levels during the embryonic and pupal stages than during the larval stages. During the larval stages it accumulates primarily in imaginal disks.

Database Links

KEGG: dme:Dmel_CG8887

STRING: 7227.FBpp0297151

UniGene: Dm.7698

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ash1 Antibody

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