AXL1 Antibody

Datasheet

Code	CSB-PA825280XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AXL1 Polyclonal antibody

Uniprot No.

Q9ZV69

Target Names

AXL1

Alternative Names

AXL1 antibody; AXL antibody; At2g32410 antibody; NEDD8-activating enzyme E1 regulatory subunit AXL antibody; Protein AXR1-LIKE antibody; Protein AXR1-LIKE 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AXL1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Regulatory subunit of the dimeric ECR1-AXL1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1 (Probable). May function redundantly with AXR1 in the RUB conjugating pathway. Seems not to be functionally equivalent to AXR1 in vivo.

Gene References into Functions

AXR1 and AXL1 differ in ability to correct phenotypic defects in axr1-30, a severe loss-of-function AXR1 mutant, when the respective coding sequences are expressed from the same promoter, suggesting differential in vivo functions. PMID: 21311953

Subcellular Location

Nucleus.

Protein Families

Ubiquitin-activating E1 family, ULA1 subfamily

Database Links

KEGG: ath:AT2G32410

STRING: 3702.AT2G32410.1

UniGene: At.38079

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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