bam Antibody

Code CSB-PA329340XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) bam Polyclonal antibody
Uniprot No.
Target Names
bam
Alternative Names
bam antibody; CG10422Protein bag-of-marbles antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) bam protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required to initiate both male and female gametogenesis. May regulate cystoblast cell divisions.
Gene References into Functions
  1. Adaptive evolution of bam has been driven at least in part by the long-term interactions between Drosophila species and Wolbachia. PMID: 26291077
  2. Our in vivo and in vitro evidence illustrate that Tut acts with Bam, Bgcn to accurately coordinate proliferation and differentiation in Drosophila germline stem cell lineage. PMID: 25412508
  3. Maintenance of female sexual identity requires the concerted actions of Sxl and bam. PMID: 25758221
  4. We show that bam repression is maintained in ote(-/-) germline stem cells and that germ cell loss persists in ote(-/-), bam(-/-) mutants. PMID: 23806619
  5. Thus, germ cells progress through distinct, sequential regulatory states, from Mei-P26 on/Bam off to Bam on/Mei-P26 off. PMID: 23122292
  6. Although Bam is important for initiating germline stem cell differentiation, its downregulation is crucial for proper spermatid terminal differentiation. PMID: 23154414
  7. Bam/Bgcn complex plays a pivotal role in promoting stem cell differentiation.[Review] PMID: 22127253
  8. the switch from proliferation to terminal differentiation is triggered by accumulation of Bam protein to a critical threshold in transit amplifying cells PMID: 20018708
  9. Repression of bag-of-marbles (bam) expression in male germline stem cells is important for male germline stem cell survival. PMID: 15238523
  10. Results describe the expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. PMID: 18423593
  11. BAM functions as a translation repressor by interfering with translation initiation. PMID: 19556547

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Tissue Specificity
In cystoblasts and/or very early cystocytes in ovary and testis.
Database Links
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