CDF1 Antibody

Code CSB-PA823820XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CDF1 Polyclonal antibody
Uniprot No.
Target Names
CDF1
Alternative Names
CDF1 antibody; DOF5.5 antibody; At5g62430 antibody; K19B1.4 antibody; MMI9.24Cyclic dof factor 1 antibody; Dof zinc finger protein DOF5.5 antibody; AtDOF5.5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CDF1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. A flanking TGT sequence contributes to the specificity of binding. Regulates a photoperiodic flowering response. Transcriptional repressor of 'CONSTANS' expression. The DNA-binding ability is not modulated by 'GIGANTEA' but the stability of CDF1 is controlled by the proteasome-dependent pathway. Ubiquitinated by the SCF(ADO3) E3 ubiquitin ligase complex. Binds to the FT promoter in the morning.
Gene References into Functions
  1. represses transcription through the TOPLESS co-repressor to control photoperiodic flowering PMID: 28752516
  2. findings show that FKF1 controls daily CONSTANS expression in part by degrading CYCLING DOF FACTOR 1 (CDF1), a repressor of CONSTANS transcription [CDF1] PMID: 16002617
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the vascular tissues of cotyledons, leaves and hypocotyls and in stomata. Not detected in roots.
Database Links

KEGG: ath:AT5G62430

STRING: 3702.AT5G62430.1

UniGene: At.9300

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