CDKA-1 Antibody

Code CSB-PA326117XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CDKA-1 Polyclonal antibody
Uniprot No.
Target Names
CDKA-1
Alternative Names
CDKA-1 antibody; CDC2 antibody; CDC2A antibody; At3g48750 antibody; T21J18.20Cyclin-dependent kinase A-1 antibody; CDKA;1 antibody; EC 2.7.11.22 antibody; EC 2.7.11.23 antibody; Cell division control protein 2 homolog A antibody; CDC2aAt antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CDKA-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Required for the function of SPCH in entering the stomatal lineage. Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 via the phosphorylation of SPCH.
Gene References into Functions
  1. CDKA;1 and CYCD3;2 are required for the terminal division in the stomatal lineage. PMID: 24687979
  2. Data indicate that the in vivo confirmation of substrates of CDKA;1 showing a direct link between cell proliferation and the control of the redox state. PMID: 22098373
  3. The crucial function of CDKA;1 is the control of the plant Retinoblastoma homolog RBR1 and codepletion of RBR1 and CDKA;1 rescued most defects of cdka;1 mutants. PMID: 22595674
  4. Expression of a dominant negative CDKA;1 allele under the control of the STM promoter perturbs post-embryonic development. Inhibition of CDK activity at the shoot apex results in premature differentiation of shoot apical meristem cells. PMID: 20659279
  5. When a single cdka;1 sperm was delivered, either female gamete could be fertilized leading to similar proportions of seeds containing either a single endosperm or a single embryo. PMID: 20610483
  6. However, we show here that the DNA damage checkpoint in Arabidopsis can also operate independently of the phosphorylation of CDKA;1. PMID: 19948791
  7. CDC2A participates in the fertilization process of endosperm PMID: 16311592
  8. The balance between cell division and differentiation is regulated through the interaction between CDKA;1 and the antiphosphatase PAS2. [CDKA;1] PMID: 16698944
  9. The Arabidopsis thaliana Cdc2(+)/Cdc28 homolog CDKA;1 is also phosphorylated in the T-loop and that phosphorylation at the conserved Thr-161 residue is essential for its function. PMID: 17369369
  10. phosphorylation of T161 in Arabidopsis CDKA;1 is essential for cell division during male gametogenesis PMID: 17764501

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Subcellular Location
Cytoplasm. Nucleus. Note=Mainly cytoplasmic. Nuclear distribution increases after binding to ICK1/KRP1.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Tissue Specificity
Expressed in roots, stems, flowers and siliques.
Database Links

KEGG: ath:AT3G48750

STRING: 3702.AT3G48750.1

UniGene: At.24166

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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