CIPK14 Antibody

Code CSB-PA885497XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CIPK14 Polyclonal antibody
Uniprot No.
Target Names
CIPK14
Alternative Names
CIPK14 antibody; PKS24 antibody; SnRK3.15 antibody; SR1 antibody; At5g01820 antibody; T20L15.90CBL-interacting serine/threonine-protein kinase 14 antibody; EC 2.7.11.1 antibody; SNF1-related kinase 3.15 antibody; SOS2-like protein kinase PKS24 antibody; Serine/threonine-protein kinase SR1 antibody; AtSR1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CIPK14 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.
Gene References into Functions
  1. Study concludes that the CIPK14 phosphorylation-mediated WHY1 deficiency in plastids is related to the impairment of protein metabolism, leading to chloroplast dysfunction PMID: 30065159
  2. SR1 acts as transcription factor in balancing plant growth and immunity. PMID: 29899210
  3. results demonstrate that CIPK14 regulates the phosphorylation and organellar distributions of WHY1 and pinpoint that CIPK14 may function as a cellular switch between leaf senescence and plastid development for coordinating the intercellular signaling in Arabidopsis PMID: 28412544
  4. the calcium-independent activation of PKS24 by SCaBP1 is dependent on interaction of PKS24 with the C-terminal tail of SCaBP1.[PKS24] PMID: 24521877
  5. yeast two-hybrid assay demonstrated that CIPK14 interacted with three CBLs and also with two key kinases PMID: 25058457
  6. AtSR1 is an important component of plant resistance to insect herbivory as well as one of only three described proteins involved in Ca(2+)/Ccalmodulin (CaM)-dependent signaling to function in the regulation of glucosinolate (GS) metabolism. PMID: 23072934
  7. Results suggest that CIPK14 plays a role in PhyA-mediated FR inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway. PMID: 21046322
  8. Induction of the gene by mono- and disaccharides and localization of expression to the leaf vasculature. PMID: 15665497
  9. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity. PMID: 18237745
  10. Comparing with wild types, the lines inserted with the T-DNA in which CIPK14 gene expression was knocked out were also more sensitive to ABA and salt stimulus, showing low germination rate and the less root elongation PMID: 18785584

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Subcellular Location
Cytoplasm. Nucleus. Note=Targeted to the tonoplast when interacting with CBL2 or CBL3 and to the cell membrane when interacting with CBL8.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Predominant in roots, cauline leaves, and flowers. Ubiquitous with highest expression in 7-day-old seedlings and flower buds, followed by that in cauline leaves and young siliques.
Database Links

KEGG: ath:AT5G01820

STRING: 3702.AT5G01820.1

UniGene: At.23738

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