Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CKI1 Polyclonal antibody

Uniprot No.

O22267

Target Names

CKI1

Alternative Names

CKI1 antibody; At2g47430 antibody; T30B22.27Histidine kinase CKI1 antibody; EC 2.7.13.3 antibody; Protein CYTOKININ-INDEPENDENT 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CKI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.

Gene References into Functions

Data suggest that the light-dependent regulation of CYTOKININ-INDEPENDENT 1 protein (CKI1) provides a plausible mechanistic link underlying the well-known interaction between light- and cytokinin-controlled plant development. PMID: 28292856
AHP2, AHP3, and AHP5 are the major factors acting downstream of CKI1 in the two-component cytokinin signaling pathway to promote Arabidopsis female gametophyte development. PMID: 28633292
The functional relevance of the conformational dynamics observed in the beta3-alpha3 loop of CKI1RD was supported by a comparison with another A. thaliana histidine kinase, ETR1. In contrast to the highly dynamic beta3-alpha3 loop of CKI1RD, the corresponding loop of the ETR1 receiver domain (ETR1RD) exhibited little conformational exchange and adopted a different orientation in crystals. PMID: 28860196
Studied sequence dependence of (13) C and (15) N chemical shifts in the receiver domain of CKI1 protein from Arabidopsis thaliana, CKI1RD , and its complexed form, CKI1RD *Mg(2+), using MD/DFT calculations. MD simulations. PMID: 26879585
CKI1-directed specification of the endosperm precursor central cell results in seeds containing an embryo and an endosperm. PMID: 27046830
The C-terminal receiver domain of ne kinase CKI1 (CKI1(RD) ) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. PMID: 21569135
CKI1-activated signaling is dependent on AHP genes which is essential for female gametophyte development. CKI1 also plays an important role during vegetative growth by regulating cell expansion. PMID: 20363773
CKI-1 is involved in cytokinin perception and demonstrates the existence of a feedback loop altering cytokinin metabolism in response to the level of receptor abundance PMID: 17602786
Crystals of the recombinant RD of the Arabidopsis thaliana HK CYTOKININ-INDEPENDENT1 (CKI1(RD)) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and glycerol as a cryoprotectant PMID: 19407381
The results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation in Arabidopsis. PMID: 19622803

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Tissue Specificity

Expressed in vascular tissues of inflorescence stems and floral organs, especially in procambium cells, and in siliques.

Database Links

KEGG: ath:AT2G47430

STRING: 3702.AT2G47430.1

UniGene: At.341

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CKI1 Antibody

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