CPC Antibody

Code CSB-PA521229XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPC Polyclonal antibody
Uniprot No.
Target Names
CPC
Alternative Names
CPC antibody; At2g46410 antibody; F11C10.10Transcription factor CPC antibody; Protein CAPRICE antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CPC protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor. Determines the fate of epidermal cell differentiation. Represses trichome development by lateral inhibition. Together with GL3 or BHLH2, promotes the formation of hair developing cells (H position) in root epidermis, probably by inhibiting non-hair cell formation. Represses the expression of GL2 and WER in H cells. Positively regulates stomatal formation in the hypocotyl.
Gene References into Functions
  1. These results indicate that the maintenance of not only the S1 and S2 motifs but also the precise structure of CPC protein might be necessary for the cell-to-cell movement of CPC. Our results should help in further unraveling of the roles of these MYB transcription factors in root hair formation PMID: 29337129
  2. that CPC, TRY and ETC1 are also involved in root hair formation at the root-hypocotyl junction. PMID: 26339713
  3. In an intricate interplay between three partly redundant single R3 MYB proteins, CPC, ETC1, and TRY regulate several developmental, physiological and metabolic processes that are putatively located in different tissues. PMID: 26022254
  4. overexpression did not influence anthocyanin biosynthesis in tomato fruit peel PMID: 26039466
  5. Double mutant analysis reveal that wer and caprice (cpc) mutants, defective in core components of the epidermal patterning pathway, genetically interact with sab. This suggests that SAB may act on epidermal patterning upstream of WER and CPC. PMID: 25124848
  6. TRY, but not the related factor CPC, is responsible for the preferential SCM accumulation. PMID: 25482776
  7. Localization of CPC in the nucleus requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3). PMID: 23832626
  8. Mutations in CPC or TRY delay flowering of cpl3 plants. A mutation in ETC1 did not further delay flowering but reduced plant size. PMID: 23796522
  9. AtMYC1 modulates the behaviour of GL1 by recruiting it from the nucleus to the cytoplasm, and AtMYC1 is recruited by TRY or CPC to the nucleus. PMID: 23900543
  10. WER and CPC compete with one another to define cell fates in the Arabidopsis root epidermis. PMID: 21914815
  11. The report adds anthocyanin biosynthesis to the set of programs that are under CPC control, indicating that this regulator is not only for developmental programs (e.g. root hairs, trichomes), but can influence anthocyanin pigment synthesis. PMID: 19825656
  12. Deletion analyses of the CPC promoter revealed that hairless cell-specific transcription of the CPC gene required a 69 bp sequence, and a tandem repeat of this region was sufficient for its expression in epidermis. PMID: 15795220
  13. Intercellular CPC movement is mediated through plasmodesmata. Furthermore, the fusion of CPC to tandem-GFPs defined the capability of CPC to increase the size exclusion limit of plasmodesmata. PMID: 16291794
  14. The functional relationship of the R2R3-type MYB gene WEREWOLF (WER) and the R3-type MYB gene CAPRICE (CPC), was analyzed. PMID: 17644729
  15. Results suggest that patterning depends on the movement of the CAPRICE and GLABRA3 transcriptional regulators between epidermal cells. PMID: 18816165

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Subcellular Location
Nucleus. Note=Moves from developing non-hair cells (atrichoblasts) to developing hair cells (trichoblasts).
Tissue Specificity
Expressed in trichomes and in young developing leaves, as well as in root hair and stele cells (pericycle and vascular tissues). Expressed in epidermal root hairless cells (atrichoblasts) and moves to root hair cells (trichoblasts) by a cell-to-cell movem
Database Links

KEGG: ath:AT2G46410

STRING: 3702.AT2G46410.1

UniGene: At.224

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