DAM1 Antibody

1. this study reports the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. PMID: 29724956
2. The authors show that the Ndc80 complex can simultaneously bind and bridge across two Dam1 complex rings through a tripartite interaction, each component of which is regulated by Aurora B kinase. PMID: 28191870
3. oligomerization of Dam1 complex is required for kinetochores to maintain microtubule attachments against tension and promote biorientation PMID: 25236177
4. Data indicate phosphorylation sites on Dam1 complex subunits. PMID: 25382489
5. aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. PMID: 25751138
6. Phosphorylation of the kinetochore protein Dam1 prevents spindle assembly checkpoint silencing when tension is absent. PMID: 24324173
7. yeast Ndc80 tail is required for timely mitotic progression and accurate chromosome segregation. The tail is essential when cells are limited for Dam1, demonstrating a redundant function for the Ndc80 and Dam1 complexes in vivo. PMID: 22851650
8. Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes PMID: 23277429
9. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore-microtubule attachment. PMID: 21965284
10. Paf1 complex and Rad6-Bre1-mediated ubiquitination of H2BK123 are required for Dam1 methylation at the kinetochore and therefore inhibit Ipl1-mediated phosphorylation, revealing unexpected functions for these proteins in mitosis. PMID: 21884933
11. The Dam1 complex is required for viability because its function as a processivity factor for kinetochore-microtubule binding is more critical when chromosome segregation is dependent upon a single kinetochore-microtubule. PMID: 21549601
12. the interaction between the Ndc80 and Dam1 complexes is abolished when the Dam1 complex is phosphorylated by the yeast aurora B kinase Ipl1. PMID: 20479468
13. Data propose that Aurora B/Ipl1-facilitated bi-orientation is stabilised in response to tension at kinetochores by dephosphorylation of Dam1, resulting in termination of kinetochore-microtubule attachment turnover. PMID: 19923271
14. The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation PMID: 16415853
15. suggested that authors have reconstituted a direct mechanism for microtubule-length control in mitosis PMID: 17572669
16. Kinetochores are tethered at the microtubule distal ends and pulled poleward as microtubules shrink (end-on pulling). The progression requires the Dam1 complex. PMID: 17620411
17. The architecture of the Dam1 complex at 30-A resolution and the self-assembly mechanism is defined. PMID: 17643123
18. Data show that rings are not necessary for dynamic microtubule attachment, or the ability of Dam1 to move processively with disassembling microtubule tips during cell division and chromosome segregation. PMID: 18364702
19. Both rings and nonencircling Dam1 oligomers can track microtubule ends and enable processive cargo movement in vitro. PMID: 18460602
20. Artificial recruitment of the microtubule-binding Dam1 complex to a plasmid lacking any centromere DNA is sufficient to confer mitotic stabilization. PMID: 19684577

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KEGG: sce:YGR113W

STRING: 4932.YGR113W