Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Dnd1 Polyclonal antibody

Uniprot No.

Q6VY05

Target Names

Dnd1

Alternative Names

Dnd1 antibody; Rbms4 antibody; TerDead end protein homolog 1 antibody; RNA-binding motif antibody; single-stranded-interacting protein 4 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Dnd1 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells. Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. Isoform 1 may play a role during primordial germ cell (PGC) survival. However, does not seem to be essential for PGC migration.

Gene References into Functions

DND1 binds a UU(A/U) trinucleotide motif predominantly in the 3' untranslated regions of mRNA, and destabilizes target mRNAs through direct recruitment of the CCR4-NOT deadenylase complex. PMID: 28297718
DND1 interacts with NANOS2 to load unique RNAs into the CCR4-NOTdeaden complex; this interaction is mediated by the zinc finger domain of NANOS2, which is essential for its association with target RNAs; DND1 is an essential partner for NANOS2 that leads to the degradation of specific RNAs in male germ cell development PMID: 26589352
Dnd1 heterozygotes, lower oxygen availability was associated with metabolic differences PMID: 26224005
results show that Dnd1 is essential for normal allelic inheritance and that Dnd1Ter has a novel combination of functions that significantly increase risk for both testicular and intestinal cancer PMID: 23773267
DND1 is the first protein known to have an RNA recognition motif directly implicated as a heritable cause of spontaneous tumorigenesis PMID: 15902260
DND1-alpha isoform to be necessary for germ cell viability and its loss in Ter mice results in germ cell depletion, germ cell tumor development and partial embryonic lethality in the 129 strain. PMID: 17291453
Trans-generational epistasis between Dnd1Ter and other modifier genes controls susceptibility to testicular germ cell tumors. PMID: 17616517
The role of dead-end in germ-cell tumor development. PMID: 17905939
when Dnd1(Ter/Ter) XX germ cells developed in a testicular environment they gave rise to the same neoplastic clusters as mutant XY germ cells in a testis. PMID: 19389346

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Involvement in disease

Defects in Dnd1 are the cause of the Ter mutation phenotype. Ter mice are characterized by primordial germ cell loss and susceptibility to spontaneous testicular germ cell tumors (TGCT). They are sterile, but viable. Isoform 1 defects may be the cause of tumor development.

Subcellular Location

Nucleus. Cytoplasm.

Tissue Specificity

Isoform 1 and isoform 2 are expressed in testis. Isoform 1 is expressed continuously in post natal (PN) testis although levels are low between PN1 to PN6. Isoform 2 is expressed from PN 20 onwards. Isoform 2 is strongly expressed in meiotic and in post-me

Database Links

KEGG: mmu:213236

STRING: 10090.ENSMUSP00000054412

UniGene: Mm.234464

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Dnd1 Antibody

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