Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DREB1A Polyclonal antibody

Uniprot No.

Q9M0L0

Target Names

DREB1A

Alternative Names

DREB1A antibody; CBF3 antibody; CRAP2 antibody; ERF072 antibody; At4g25480 antibody; M7J2.150Dehydration-responsive element-binding protein 1A antibody; Protein DREB1A antibody; C-repeat/dehydration-responsive element-binding factor 3 antibody; C-repeat-binding factor 3 antibody; CRT/DRE-binding factor 3 antibody; Cold resistance-related AP2 transcription factor antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DREB1A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.

Gene References into Functions

The Arabidopsis thaliana gene DREB1A/CBF3, encoding a stress-inducible transcription factor. Ectopic expression of AtDREB1A in Salvia miltiorrhiza resulted in increased drought tolerance and increased photosynthetic rate when subjected to drought stress. PMID: 27485523
These results indicate the implicit influence of rd29A::DREB1A on mechanisms underlying water uptake, stomatal response, transpiration efficiency and rooting architecture in water-stressed plants. PMID: 25326370
Potato plants ectopically expressing AtCBF3 exhibited enhanced tolerance to high temperature, which is associated with improved photosynthesis and antioxidant defence via induction of the expression of many stress-inducible genes. PMID: 24811248
The physiological studies revealed that the expression of AtDREB1A was associated with an increased accumulation of the osmotic substance proline, maintenance of chlorophyll, increased relative water content and decreased ion leakage under drought stress. PMID: 24398893
Stress-inducible over-expression of Arabidopsis CBF3 gene may have the potential to enhance abiotic stress tolerance in oat. PMID: 22325896
The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean. PMID: 23029105
Overexpression of AtDREB1A in soybean appears to enhance drought tolerance. PMID: 22033903
SOC1 Directly Represses the Expression of CBF3 Genes. PMID: 19825833
The recombinant DREB1A protein bound to A/GCCGACNT more efficiently than to A/GCCGACNA/G/C. PMID: 15165189
Makes transgenic plants more tolerant to stress conditions. PMID: 15834008
Regulon genes repressed by siz1 did not affect expression of ICE1, which encodes a MYC transcription factor that controls CBF3/DREB1A. PMID: 17416732
The freezing tolerance of 38 independent transgenic potato lines was tested in vitro using plantlets transgenic for the DREB1A gene under control of the rd29A promoter. PMID: 17453213
Overexpression of DREB1A improves stress tolerance to both freezing and dehydration while overexpression of an active form of DREB2A results in significant stress tolerance to dehydration but only slight tolerance to freezing. PMID: 19502356

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Subcellular Location

Nucleus.

Protein Families

AP2/ERF transcription factor family, ERF subfamily

Database Links

KEGG: ath:AT4G25480

STRING: 3702.AT4G25480.1

UniGene: At.231

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