DREB2A Antibody

Code CSB-PA525747XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DREB2A Polyclonal antibody
Uniprot No.
Target Names
DREB2A
Alternative Names
DREB2A antibody; ERF045 antibody; At5g05410 antibody; K18I23.22Dehydration-responsive element-binding protein 2A antibody; Protein DREB2A antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DREB2A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription.
Gene References into Functions
  1. Genetic analysis indicated that the depletion of BTB/POZ AND MATH DOMAIN protein expression conferred enhanced thermotolerance via DREB2A stabilization PMID: 28923951
  2. DPB3-1 associates with NF-YA and NF-YB subunits to enhance heat stress-inducible gene expression during heat stress responses in cooperation with DREB2A. PMID: 25490919
  3. The stabilization of DREB2A is important but not sufficient to induce target gene expression; further activation processes are required. PMID: 24376497
  4. The stress-inducible expression of Arabidopsis thaliana DREB2A CA in transgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. PMID: 24656336
  5. GRF7 binds to a region of the DREB2A promoter and represses its activity. PMID: 22942381
  6. Functional interactions between Dreb2a and Med25 induce conformational changes which in turn cause changes in their binding affinity. PMID: 22447446
  7. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions. PMID: 22150398
  8. These results suggest that in addition to an abscissic acid (ABA)-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A. PMID: 22025559
  9. The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation. PMID: 16617101
  10. Functions in both water-stress and heat shock-stress responses. PMID: 17030801
  11. Altogether these data demonstrate that HsfA3 is transcriptionally controlled by DREB2A and important for the establishment of thermotolerance. PMID: 17999647
  12. These results indicate that HsfA3 regulates expression of many heat-inducible genes in the transcriptional cascade downstream of the DREB2A stress-regulatory system and functions in acquisition of thermotolerance under the control of the DREB2A cascade. PMID: 18261981

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed preferentially in roots and stems, and at a lower level in leaves.
Database Links

KEGG: ath:AT5G05410

STRING: 3702.AT5G05410.1

UniGene: At.25247

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