E4 Antibody

Datasheet

Code	CSB-PA356968ZA01HML
Size
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Human papillomavirus type 16 E4 Polyclonal antibody

Uniprot No.

P06922

Target Names

Alternative Names

Protein E4 (E1^E4), E4

Raised in

Rabbit

Species Reactivity

Human papillomavirus type 16

Immunogen

Recombinant Human papillomavirus type 16 E4 protein

Immunogen Species

Human papillomavirus type 16

Conjugate

Non-conjugate

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Contributes to multiple aspects of the viral life cycle including viral genome amplification, suppression of suprabasal cell differentiation and egress of newly formed virions. Induces host cell cycle arrest at the G2 phase by associating with and preventing the nuclear entry of host CDK1/cyclin B1 complexes. Inhibits cellular DNA replication by preventing loading of host replication licensing proteins MCM2 and MCM7 onto chromatin. Within the cytoplasm, associates with host kinase SRPK1, a splicing factor regulator, and inhibits its activity. Therefore, E4 favors expression of late viral transcripts by inhibiting SRPK1-mediated phosphorylation of host serine-arginine (SR) proteins that have critical roles in mRNA metabolism. Late in the infectious cycle, E4 also acts to diminish the integrity of the keratinocyte by disrupting the keratin cytoskeleton and inducing apoptosis through alteration of mitochondrial function to facilitate egress of the newly formed virions.

Gene References into Functions

These results suggest that 16E4-mediated enhancement of genome amplification involves its cell cycle inhibition and cellular kinase activation functions, with E4 modifying the activity and function of viral replication proteins including E1 PMID: 28306742
Expression of the HPV16 E4 and E5 proteins results in a substantial change in the composition of the cell membrane and extracellular space, associated with alterations in cell adhesion and differentiation. PMID: 24898764
20 cervical cyology samples with HPV16 were tested by RT-PCR for HPV16 E4 mRNA, found in sqaumous intraepithelial lesion and squamous carcinoma, but not in samples negative for intrepithelial lesion. E4 expression assay may be useful in cancer diagnosis. PMID: 22299437
The data suggest a model in which the expression of 16E5 from the major E1--E4-E5 mRNA promotes T57 phosphorylation of E1--E4 by ERK and keratin binding. PMID: 19211765
identified the N-terminus of E2 as the first example of a viral protein that directly binds the E1--E4 protein PMID: 19783272

Hide All

Subcellular Location

Host cytoplasm. Host nucleus.

Protein Families

Papillomaviridae E4 protein family

Database Links

KEGG: vg:1489076

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

GCGR: A Regulator of Energy Balance Holds a Potential in Treatment Diabetes CD73, What a Powerful Therapy Target in Tumor Immune Exploring Biological Landscape of Cervical Cancer: A Comprehensive Overview for Researchers A Comprehensive Overview of Ovarian Cancer The Overview of Fc Receptors- Classification, Related Signaling Pathways and Application The Overview of Cell Viability Exploring Neurodegeneration: A Comprehensive Guide for Researchers CD70 (CD27L/TNFSF7): a TNF Receptor Member, a “Star” Target for Clinical Research in Systemic Lupus Erythematosus (SLE), Arthritis, and Tumors! LY6G6D (LY6-G): A New Member of Lymphocyte Antigen-6 Family Holds Big Opportunities in Bispecific Antibody for CRC Treatment! Mucin-17/MUC17: An Unexpected Target for Tumor Immune Therapy, Notably Gastric Cancer! The Overview of Interleukin IGFL1: A Novel Secretory Protein of IGF-Like Family Presents a High Cancer-Promoting Potential! CADM1: an Immunoglobulin Superfamily Molecule, a New Tumor Suppressor Gene! MAGEA4: a Member of the Melanoma-Associated Antigen (MAGE) Family, a Popular Target for Tumor-Specific Immune Research! Dipeptidase 3 (DPEP3): an Emerging Member of the DPEP Family, a Potential Therapeutic Target in Male Fertility or Tumor Research! Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

E4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background