EB1B Antibody

Code CSB-PA579570XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EB1B Polyclonal antibody
Uniprot No.
Target Names
EB1B
Alternative Names
EB1B antibody; At5g62500 antibody; K19B1.11Microtubule-associated protein RP/EB family member 1B antibody; APC-binding protein EB1B antibody; End-binding protein 1 antibody; AtEB1 antibody; End-binding protein 1B antibody; AtEB1B antibody; Protein ATEB1 homolog 2 antibody; ATEB1H2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EB1B protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. May be involved in anchoring microtubules to their nucleation sites and/or functioning as a reservoir for distribution to the growing end. In plants, microtubule minus ends are not necessarily severed from the nucleation site and transported to the plus end of a microtubule as part of the recycling process. May play a role in endomembrane organization during polarized growth of plant cells.
Gene References into Functions
  1. eb1b-1 mutant roots exhibit an increase over wild type in their response to touch... Mutant roots are also hypersensitive to increased levels of mechanical stimulation, revealing the presence of another process that activates the response. PMID: 22404827
  2. The results suggest that a plant-specific EB1 subtype has evolved to function preferentially on the spindle microtubules by accumulating in the prophase nucleus. PMID: 20067996
  3. Data find that antibodies directed against EB1 proteins colocalize with microtubules in roots, and that mutants with reduced expression from EB1B genes have roots that deviate toward the left on vertical or inclined plates. PMID: 18281505
Subcellular Location
Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, phragmoplast. Note=Localizes both to the plus ends of microtubules and to the sites of nucleation during division and interphase.
Protein Families
MAPRE family
Tissue Specificity
Highly expressed in guard cells of leaf stomata, pollen grains and pollen tubes. Expressed in young roots.
Database Links

KEGG: ath:AT5G62500

STRING: 3702.AT5G62500.1

UniGene: At.29046

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