EC1.1 Antibody

Code CSB-PA273066XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EC1.1 Polyclonal antibody
Uniprot No.
Target Names
EC1.1
Alternative Names
EC1.1 antibody; At1g76750 antibody; F28O16.12Egg cell-secreted protein 1.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EC1.1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the regulation of gamete interactions during the double fertilization and to prevent multiple-pollen tube attraction; mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell surface after secretion upon sperm arrival.
Gene References into Functions
  1. The egg cell secretes EC1 proteins upon sperm arrival to promote rapid sperm activation, thereby accelerating gamete fusion and preventing polytubey.[EC1.1] PMID: 24384993
Subcellular Location
Cytoplasmic vesicle. Secreted. Note=Secreted via vesicle exocytose upon sperm arrival, especially in the apical region of the degenerating synergid cell.
Protein Families
Plant egg cell-secreted peptide family
Tissue Specificity
Restricted to female reproductive tissues, specifically accumulating in storage vesicles of the unfertilized egg cell.
Database Links

KEGG: ath:AT1G76750

STRING: 3702.AT1G76750.1

UniGene: At.34618

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