EDM2 Antibody

Datasheet

Code	CSB-PA189255XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EDM2 Polyclonal antibody

Uniprot No.

F4K3G5

Target Names

EDM2

Alternative Names

EDM2 antibody; At5g55390 antibody; MTE17.10Protein ENHANCED DOWNY MILDEW 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EDM2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Cellular antisilencing factor and regulator of genome DNA methylation patterns involved in the regulation of chromatin states. Together with SUVH4, monitors repressive epigenetic marks H3K27me1, H3K9me2, and prevents DNA-methylation at CHG sites, affecting especially the expression of transposons and developmentally important genes. Regulates alternative RNA processing such as distal 3' polyadenylation by intronic heterochromatin. Transcription factor that binds DNA and contributes to transcriptional transposable element (TE) silencing by modulating levels of the repressive post-translational histone modifications (PHM) H3K9me2. In cv. Columbia, required for RPP7-dependent disease resistance against the Hyaloperonospora arabidopsidis isolate Hiks1, by promoting levels of RPP7 via alternative polyadenylation (APA), resulting from cooption of epigenetic information at the TE insertion locus COPIA-R7. Regulates development processes such as the formation of leaf pavement cells, leaf expansion, fertility and flowering. Prevents FLC accumulation to control flowering.

Gene References into Functions

EDM2 promotes vegetative leaf growth and leaf pavement cell morphology, and is modulated by WNK8. PMID: 20840782
The role of EDM2 in plant immunity is limited and does not include a function in basal defense. EDM2 acts upstream of the floral repressor FLC and downstream of WNK8 to affect floral transition. PMID: 20149132
In edm2 mutants both constitutive and Hyaloperonospora parasitica isolate Hiks1-induced RPP7 transcript levels are reduced, suggesting that EDM2 is either a direct or an indirect regulator of RPP7 expression. PMID: 17253987

Subcellular Location

Nucleus.

Database Links

KEGG: ath:AT5G55390

STRING: 3702.AT5G55390.1

UniGene: At.25531

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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