Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EDR1 Polyclonal antibody

Uniprot No.

Q9FPR3

Target Names

EDR1

Alternative Names

EDR1 antibody; STY10 antibody; At1g08720 antibody; F22O13.20Serine/threonine-protein kinase EDR1 antibody; EC 2.7.11.1 antibody; MAPKK kinase EDR1 antibody; Protein ENHANCED DISEASE RESISTANCE 1 antibody; AtEDR1 antibody; Serine/threonine/tyrosine-protein kinase 10 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EDR1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

MAPKKK serine/threonine-protein kinase involved in the regulation of a MAP kinase cascade (probably including MPK3 and MPK6) that negatively regulates salicylic acid- (SA-) dependent defense responses, abscisic acid (ABA) signaling, and ethylene-induced senescence. Modulates also stress response (e.g. drought) signaling and cell death, in an ORE9-dependent manner. Functions at a point of cross talk between ethylene, ABA and SA signaling that impinges on senescence and cell death. On the other hand, it confers sensitivity to various pathogens such as the fungus E.cichoracearum, the oomycete H.parasitica and the bacteria P.syringae pv. tomato DC3000. Required for resistance to some hemibiotrophic/necrotrophic fungal pathogens (e.g. C.gloeosporioides, C.higginsianum and A.brassicicola) through the induction of defensin expression, probably by repressing MYC2, an inhibitor of defensin genes (PDFs). Together with KEG, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.

Gene References into Functions

EDR4 associates with CHC2 and modulates plant immunity by regulating the relocation of EDR1 in Arabidopsis. PMID: 25747881
ATL1 is a positive regulator of programmed cell death and EDR1 negatively regulates ATL1 activity at the TGN/EE and thus controls stress responses initiated by ATL1-mediated ubiquitination events. PMID: 25398498
A natural edr1 mutant in the background of C24, was identified. PMID: 24925473
It was concluded that EDR1 contributes to the defense response of A. thaliana against P. infestans; these data position EDR1 as a negative regulator in postinvasive nonhost resistance. PMID: 25353364
A catalytically inactive form of the EDR1 kinase domain was successfully crystallized in the presence of the ATP analogue AMP-PNP. PMID: 25005098
EDR1 functions as a positive regulator of non-host resistance independently of PEN2, and is critical for pathogen-induced expression of plant defensins. PMID: 22057322
EDR1 exerts a positive and critical role in resistance responses to hemibiotrophic/necrotrophic fungi, in part by inducing antifungal protein expression through derepression of MYC2 function. PMID: 21605210
The expression of many known and putative defence-associated genes was more rapidly induced, and to higher levels, in edr1 plants relative to the wild-type PMID: 21726375
EDR1 exerted negative regulation on HR cell death and powdery mildew resistance by limiting the transcriptional amplification of RPW8.1 and RPW8.2. PMID: 15773856
The edr1 mutant displays enhanced stress responses and spontaneous necrotic lesions under drought conditions in the absence of pathogen, suggesting that EDR1 is also involved in stress response signaling and cell death regulation. PMID: 15894742
conclude that edr1-mediated resistance to powdery mildew is mediated, in part, by enhanced ABA signaling PMID: 18815384

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Subcellular Location

Cell membrane. Endosome. Nucleus. Endoplasmic reticulum. Golgi apparatus, trans-Golgi network. Early endosome.

Protein Families

Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily

Database Links

KEGG: ath:AT1G08720

STRING: 3702.AT1G08720.1

UniGene: At.20614

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EDR1 Antibody

Product Details

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Target Background