tufA Antibody

Code CSB-PA025342XA01EOD
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 tufA Polyclonal antibody
Uniprot No.
Target Names
tufA
Alternative Names
tufA antibody; Z4697 antibody; ECs4190; antibody; tufB antibody; Z5553 antibody; ECs4903Elongation factor Tu antibody; EF-Tu antibody; P-43 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 tufA protein
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.; May play an important regulatory role in cell growth and in the bacterial response to nutrient deprivation.
Gene References into Functions
  1. Authors studied the transition between the pre- and post-hydrolysis structures through two distinct pathways and show that the transition free energy is minimal along a non-intuitive pathway that involves "separation" of the GTP binding domain (domain 1) from the OB folds (domains 2 and 3), followed by domain 1 rotation, and, eventually, locking the EF-Tu conformation in the post-hydrolysis state. PMID: 29045140
Subcellular Location
Cytoplasm. Cell inner membrane; Peripheral membrane protein.
Protein Families
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily
Database Links

KEGG: ece:Z4697

STRING: 155864.Z4697

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