ENDO1 Antibody

Code CSB-PA953884XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ENDO1 Polyclonal antibody
Uniprot No.
Target Names
ENDO1
Alternative Names
ENDO1 antibody; BFN1 antibody; At1g11190 antibody; T28P6.14Endonuclease 1 antibody; AtENDO1 antibody; EC 3.1.30.1 antibody; Bifunctional nuclease I antibody; AtBFN1 antibody; Deoxyribonuclease ENDO1 antibody; Single-stranded-nucleate endonuclease ENDO1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ENDO1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Endonuclease that can use RNA, single-stranded and double-stranded DNA as substrates. Hydrolyzes single-stranded DNA and RNA without apparent specificity for bases during senescence. Endonuclease that recognizes and cleaves all types of mismatches with high efficiency, including heteroduplex double-stranded DNA. Maybe involved in programmed cell death (PCD) and senescence.
Gene References into Functions
  1. BFN1 (ENDO1) is a Ca2+/Mn2+-dependent nuclease, acting at neutral pH. PMID: 23620482
  2. localization of BFN1 PMID: 21665915
  3. ENDO1 detects and cleaves double-stranded DNA at point mutations with high specificity. PMID: 17651368
  4. BFN1 is involved in programmed cell death associated with different development processes in Arabidopsis. PMID: 18603613
Protein Families
Nuclease type I family
Tissue Specificity
Mostly expressed in flowers and during leaf and stem senescence, and, to a lower extent, detectable at low levels in roots, leaves, and stems. Particularly expressed in senescing tissues in a NAC92/ORE1-dependent manner.
Database Links

KEGG: ath:AT1G11190

STRING: 3702.AT1G11190.1

UniGene: At.65036

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