Code | CSB-PA318126ZA01ENL |
Size | US$299 |
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The production of the F17a-A polyclonal antibody follows a systematic protocol. It begins with the repeated immunization of a rabbit using recombinant Escherichia coli F17 fimbrial protein (F17a-A) (22-180aa) until an optimal antibody titer is achieved. Afterward, the rabbit's blood is collected, and the antibodies are meticulously purified from the serum using protein A/G. The functionality of the resulting F17a-A antibody is extensively assessed through ELISA and WB applications, confirming its specific reactivity with the Escherichia coli F17a-A protein.
F17a-A is one of the variants of the F17A, the major subunit of the F17 fimbriae. F17 fimbriae is one of the most prevalent and crucial virulence factors among the pathogenic Escherichia coli isolated from diarrheic and septicemic animals of various species. F17a-A is associated with adherence to host cells in the intestines and is important for the colonization and infection of animals.
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