GID2 Antibody

Code CSB-PA890438XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GID2 Polyclonal antibody
Uniprot No.
Target Names
GID2
Alternative Names
GID2 antibody; SLY1 antibody; At4g24210 antibody; T22A6.40F-box protein GID2 antibody; Protein SLEEPY 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GID2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential component of the SCF-type E3 ligase complex, SCF(GID2), a complex that positively regulates the gibberellin signaling pathway. Upon gibberellin treatment, the SCF(GID2) complex mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2), some repressors of the gibberellin pathway, leading to activate the pathway.
Gene References into Functions
  1. results indicate that, in Arabidopsis, AtSIZ1 positively controls GA signalling through SLY1 sumoylation. PMID: 26008766
  2. SNE function partly overlaps with SLY1 function in gibberellin signaling. PMID: 21163960
  3. SLY1 interacts directly with RGA and GA INSENSITIVE (GAI, a closely related DELLA protein) via their C-terminal GRAS domain PMID: 15155881
  4. SLY1 (the wild-type form) and sly1gar2-1 both confer substrate specificity on this complex via specific binding to the DELLA proteins PMID: 15161962
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in all tissues tested, including rosette leaves, green siliques, flowers, stems, cauline leaves and seedlings.
Database Links

KEGG: ath:AT4G24210

STRING: 3702.AT4G24210.1

UniGene: At.43332

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