GIG1 Antibody

Code CSB-PA787588XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GIG1 Polyclonal antibody
Uniprot No.
Target Names
GIG1
Alternative Names
GIG1 antibody; EBO30 antibody; OSD1 antibody; UVI4-LIKE antibody; At3g57860 antibody; T10K17.70Protein GIGAS CELL1 antibody; Protein ENHANCER OF BON1-2 30 antibody; Protein OMISSION OF SECOND DIVISION1 antibody; Protein UV-B-INSENSITIVE 4-like antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GIG1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negative regulator of the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase required for proper mitotic and meiotic progression and cell fate determination. Involved in entry into both meiosis I and meiosis II. Prevents endomitosis by preferentially inhibiting APC/C(CDC20). Required for megagametophyte and endosperm development. Triggers mitotic cyclins (e.g. CYCB1-1 and CYCB1-2) accumulation. Confers immunity to bacterial pathogens (e.g. Pseudomonas syringae pv. tomato DC3000), which is associated with increased expression of disease resistance (R) genes. GIG1 and PANS1 are part of a network linking centromere cohesion and cell cycle progression through control of APC/C activity.
Gene References into Functions
  1. A functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor. [OSD1] PMID: 22844260
  2. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor. PMID: 22844260
  3. Combining tam and osd1 mutations leads to a failure in the prophase to meiosis I transition during male meiosis and to the production of tetraploid spores and gametes. [OSD1] PMID: 20585549
Tissue Specificity
Expressed in rapidly dividing tissues such as shoot apical meristem and young leaves. Associated with cell division but also with specific cell types.
Database Links

KEGG: ath:AT3G57860

STRING: 3702.AT3G57860.1

UniGene: At.21178

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