GL1 Antibody

Code CSB-PA340923XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GL1 Polyclonal antibody
Uniprot No.
Target Names
GL1
Alternative Names
GL1 antibody; MYB0 antibody; At3g27920 antibody; K16N12.17 antibody; Trichome differentiation protein GL1 antibody; Myb-related protein 0 antibody; AtMYB0 antibody; Protein GLABRA 1 antibody; Protein GLABROUS 1 antibody; R2R3-MYB transcription factor GL1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GL1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator, when associated with BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate specification in leaves. Together with TTG1 and GL3, promotes trichome formation and endoreplication. Regulates the production of a signal that induces hair (trichome) precursor cells on leaf primordia to differentiate. Binds to the WER-binding sites (WBS) promoter regions and activates the transcription of target genes.
Gene References into Functions
  1. These results suggested that the Serine 92 in the conserved [D/E]Lx2 [R/K]x3Lx6Lx3R amino acid signature of GL1 is not essential for the interaction of GL1 and GL3, but may play a role in the binding of GL1 to the promoters of its target genes. PMID: 26667588
  2. GL1 represses the activation of the TRY promoter by GL3 and TTG1, and TTG1 suppresses the activation of the CPC promoter by GL1 and GL3. PMID: 25926482
  3. Protein interaction analysis revealed that MYB82 physically interacts with GLABRA3. In addition, MYB82 and GL1 can form homodimers and heterodimers at R2R3-MYB domains, which may explain why their overexpression reduces trichome numbers. PMID: 24803498
  4. AtMYC1 protein is predominantly localised in the cytoplasm and can relocate GL1 from the nucleus into the cytoplasm. PMID: 23900543
  5. GL1 has qualitative and likely quantitative effects on trichome density in natural populations of Arabidopsis thaliana. PMID: 22625421
  6. Impaired fatty acid/lipid flux is associated with a nonallelic GLABRA1 mutation which causes abnormal cuticle formation and impaired plant immunity. PMID: 20699396
  7. TRICHOMELESS1 can be recruited to the cis-acting regulatory elements of GLABRA1 PMID: 17933793
  8. Data show that GL1 associates with TRANSPARENT TESTA GLABRA1 in vivo, forming a complex. PMID: 18434419
  9. DNA binding of GL1 and GL3 via their own DNA-binding domains to the promoter of GL2 is probably required for the activation of GL2 expression. PMID: 18948276
  10. 20 novel genes in the regulatory network involved in the trichome formation are the direct targets of GL3/GL1. PMID: 19247443

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Subcellular Location
Nucleus. Note=Detected in trichome nucleus.
Tissue Specificity
Expressed in leaves, stems and flowers. Expressed in trichome cells and in leaf primordia.
Database Links

KEGG: ath:AT3G27920

STRING: 3702.AT3G27920.1

UniGene: At.42881

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