GLK1 Antibody

Code CSB-PA882872XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GLK1 Polyclonal antibody
Uniprot No.
Target Names
GLK1
Alternative Names
GLK1 antibody; GPRI1 antibody; At2g20570 antibody; F23N11.11 antibody; Transcription activator GLK1 antibody; GBF'S PRO-RICH REGION-INTERACTING factor 1 antibody; Golden2-like protein 1 antibody; AtGLK1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GLK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that functions with GLK2 to promote chloroplast development. Acts as an activator of nuclear photosynthetic genes involved in chlorophyll biosynthesis, light harvesting, and electron transport. Acts in a cell-autonomous manner to coordinate and maintain the photosynthetic apparatus within individual cells. May function in photosynthetic capacity optimization by integrating responses to variable environmental and endogenous cues. Prevents premature senescence.
Gene References into Functions
  1. P69 binding to GLK proteins may interfere with the interaction between GLK proteins and upstream transcription factors, causing defective binding of GLK proteins to their targets. PMID: 27964999
  2. silencing of AtRAP by AtlsiRNA-1 upon bacterial infection triggers defense responses through regulation of LSU2 and GLK1. PMID: 28656601
  3. Plastid signals down-regulate the accumulation of GLK1 through the ubiquitin-proteasome pathway. PMID: 27821720
  4. Overexpressors of the Golden2-like transcription factors GLK1 and GLK2 were identified as genomes uncoupled mutants. PMID: 26876646
  5. plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone PMID: 27035938
  6. data reveal that AtGLK1 is involved in JA-dependent susceptibility to the biotrophic pathogen Hpa Noco2 and in JA-independent resistance to the necrotrophic pathogen B. cinerea PMID: 24393452
  7. Overexpression of GLKs caused up-regulation of not only their direct targets but also non-target nuclear and plastid genes, leading to global induction of chloroplast biogenesis in the root. PMID: 23749810
  8. ORE1 interacts with the G2-like transcription factors GLK1 and GLK2, which are important for chloroplast development and maintenance. PMID: 23459204
  9. AtGLK1 may coordinate plastid protein import and nuclear gene expression. PMID: 19726569
  10. GLK1 'regulon' encodes disease defense related proteins and confers resistance to Fusarium graminearum in Arabidopsis PMID: 17533111
  11. GLK1 and GLK2 are functionally equivalent. They act in a cell-autonomous manner to regulate chloroplast development. When mis-expressed in the phloem, GLKs can be unloaded into adjoining photosynthetic tissue. PMID: 18643989
  12. GLK1 and GLK2 stimulate expression of chlorophyll biosynthesis and light-harvesting genes. PMID: 19376934

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in rosette and cauline leaves. Expressed at low levels in cotyledons and shoots.
Database Links

KEGG: ath:AT2G20570

STRING: 3702.AT2G20570.2

UniGene: At.24134

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