Gnai3 Antibody

Code CSB-PA009591XA01RA
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Gnai3 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
Gnai3 antibody; Gnai-3Guanine nucleotide-binding protein G(i) subunit alpha antibody; G(i) alpha-3 antibody
Raised in
Rabbit
Species Reactivity
Rattus norvegicus (Rat)
Immunogen
Recombinant Rattus norvegicus (Rat) Gnai3 protein
Immunogen Species
Rattus norvegicus (Rat)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Gene References into Functions
  1. Nitric oxide decreased the enhanced expression of Gialpha-2/3 proteins and hyperproliferation of Vascular smooth muscle cells from spontaneously hypertensive rats by cGMP-independent mechanism. PMID: 28692698
  2. These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins. PMID: 24155894
  3. Results suggest that the suppression of Dopamine-2 (D2) receptor short (D2S)-regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling estradiol (E(2)-activated prolactin (PRL) synthesis and cell proliferation in lactotropes. PMID: 22573829
  4. studies suggest a possible role for zymogen granule-associated G(alpha)(i3) in zymogen granule-plasma membrane fusion PMID: 12038977
  5. Suggest role for oxidative stress in high glucose-induced decreased expression of Gnai2/3 and adenylyl cyclase signaling in vascular smooth muscle cells. PMID: 18441196

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Subcellular Location
Cytoplasm. Cell membrane; Lipid-anchor. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
G-alpha family, G(i/o/t/z) subfamily
Tissue Specificity
Ubiquitous.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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