Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HO1 Polyclonal antibody

Uniprot No.

O48782

Target Names

HO1

Alternative Names

HO1 antibody; GUN2 antibody; HY1 antibody; HY6 antibody; TED4 antibody; At2g26670 antibody; F18A8.4Heme oxygenase 1 antibody; chloroplastic antibody; AtHO1 antibody; EC 1.14.14.18 antibody; Protein GENOMES UNCOUPLED 2 antibody; Protein REVERSAL OF THE DET PHENOTYPE 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HO1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Key enzyme in the synthesis of the chromophore of the phytochrome family of plant photoreceptors. Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Can form complex with heme, is ferredoxin-dependent and its activity is increased in the presence of ascorbate. Plays a role in salt acclimation signaling. May affect the plastid-to-nucleus signaling pathway by perturbing tetrapyrrole synthesis. The plastid-to-nucleus signal plays an important role in the coordinated expression of both nuclear- and chloroplast-localized genes that encode photosynthesis-related proteins.

Gene References into Functions

Data indicate that Heme oxygenase 1 (HY1) functioned negatively and acted upstream of ABSCISIC ACID-INSENSITIVE4 (ABI4) in drought signaling. PMID: 26704641
HY1 confers cadmium tolerance by decreasing nitric oxide production and improving iron homeostasis. PMID: 23974911
Data indicate that AtHO1 (HY1; heme oxygenase-1)-overexpressing plants generated more NO, whereas knock-down of AtHO1 expression reduced the level of nitric oxide (NO) in plants. PMID: 23620481
HY1 mutant exhibited progressive salt hypersensitivity. PMID: 23744476
Disrupting the binding of the AtHBP5 to haem oxygenase 1 (HY1) leads to oxidative stress. PMID: 22991161
Mutation of HY1 causes UV-C hypersensitivity by impairing carotenoid and flavonoid biosynthesis and the down-regulation of antioxidant defence. PMID: 22419743
HY1 and HY5 additively regulate the expression of light regulated genes and accumulation of chlorophyll and anthocyanin during early seedling development. PMID: 22424472
HY1 (Heme oxygenase 1) plays an important role in salt acclimation. PMID: 21205037
all members of the HO1 subfamily (HY1, HO3 and HO4) are active monomeric HOs and can convert haem to BV IXalpha using spinach Fd (ferredoxin) as an electron donor PMID: 19860740

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Subcellular Location

Plastid, chloroplast.

Protein Families

Heme oxygenase family

Tissue Specificity

Widely expressed.

Database Links

KEGG: ath:AT2G26670

STRING: 3702.AT2G26670.1

UniGene: At.23645

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HO1 Antibody

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