HTR12 Antibody

Code CSB-PA840421XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HTR12 Polyclonal antibody
Uniprot No.
Target Names
HTR12
Alternative Names
HTR12 antibody; CENH3 antibody; At1g01370 antibody; F6F3.17Histone H3-like centromeric protein HTR12 antibody; Centromeric histone CENH3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HTR12 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
Gene References into Functions
  1. Extreme divergence in CENH3 protein sequence does not alter centromere location in Arabidopsis. PMID: 28223399
  2. Reduced endogenous CENH3 and lack of meiotic incorporation of EYFP-CENH3(C) lead to reduced fertility caused by insufficient CENH3 loading to the centromeres of meiotic chromosomes, subsequent lagging of chromosomes and formation of micronuclei. PMID: 21635586
  3. CENH3 is expressed in dividing tissues and is CENH3 is transcriptionally regulated by E2F/DP transcription factors. PMID: 21771121
  4. Deposition of Arabidopsis CENH3 takes place at centromeres during G2. PMID: 21842230
  5. Meiosis-specific loading of the centromere-specific histone CENH3 in Arabidopsis thaliana PMID: 21695238
  6. study concludes that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis PMID: 20628040
  7. Alignment of CENH3 sequences revealed that the tested species share only three amino acids at loop1 region: threonine2, arginine12 and alanine15 PMID: 21190064
  8. cenh3 null mutants expressing altered CENH3 proteins are crossed to wild type, chromosomes from the mutant are eliminated, producing haploid progeny PMID: 20336146

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Subcellular Location
Chromosome, centromere, kinetochore.
Protein Families
Histone H3 family
Database Links

KEGG: ath:AT1G01370

STRING: 3702.AT1G01370.1

UniGene: At.42652

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