Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HYH Polyclonal antibody

Uniprot No.

Q8W191

Target Names

HYH

Alternative Names

HYH antibody; BZIP64 antibody; At3g17609 antibody; MKP6.27Transcription factor HY5-like antibody; HY5 homolog antibody; bZIP transcription factor 64 antibody; AtbZIP64 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HYH protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes.

Gene References into Functions

It has been reported that HY5 regulates HYH expression in roots and contributes to root growth under different light conditions. PMID: 28683099
light affected TCP2 expression in a wavelength-dependent manner and TCP2 positively regulates mRNA expression of HYH and HY5. PMID: 26596765
This study further reveals that MYC2 and GBF1 bind to HYH promoter and inhibit each other through non-DNA binding bHLH-bZIP heterodimers. PMID: 26047210
The DNA binding activity of GBF1 is modulated by heterodimeriation with HY5 and HYH. PMID: 24157608
BBX24 and BBX25 physically interact with HYH. PMID: 23733077
the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. PMID: 22692212
light triggers MSBP1 expression through direct binding to and activation by HY5 and HYH. PMID: 21715650
HY5 and HYH are two necessary regulators that play a pivotal role during low temperature-induced anthocyanin accumulation in Arabidopsis seedlings. PMID: 20932601
Together with HY5/HYH, gibberellins signaling may play an important role during low temperature-induced anthocyanin accumulation. PMID: 21636970
HY5 and HYH were activators of NIA2, but inhibitors of NRT1.1 when tested across various light treatments and tissue types. PMID: 19540016
The data support the hypothesis that the opposite root system phenotypes of hy5 single and hy5 hyh double mutants represent the morphological response to a quantitative gradient in the same molecular process. PMID: 17121469
important for high nitrate reductase expression in red light PMID: 17929051
This research provides evidence that, in ultraviolet B (UV-B) signaling, UVR8, HY5, and HYH act together and demonstrates a new role for HYH in UV-B responses. PMID: 18055587
HYH and PEX11b constitute a novel branch of the phyA-mediated light signaling cascade, which promotes peroxisome proliferation during seedling photomorphogenesis. PMID: 18203870

Hide All

Subcellular Location

Nucleus.

Protein Families

BZIP family

Database Links

KEGG: ath:AT3G17609

STRING: 3702.AT3G17609.2

UniGene: At.28302

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HYH Antibody

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