IFH1 Antibody

Code CSB-PA340112XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) IFH1 Polyclonal antibody
Uniprot No.
Target Names
IFH1
Alternative Names
IFH1 antibody; RRP3 antibody; YLR223C antibody; L8083.9 antibody; Protein IFH1 antibody; Ribosomal RNA-processing protein 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) IFH1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Controls the pre-rRNA processing machinery in conjunction with FHL1. Could convert FHL1 from a repressor to an activator.
Gene References into Functions
  1. The role of CK2-dependent phosphorylation of Ifh1 and Crf1 in transcriptional regulation of ribosomal protein genes in Saccharomyces cerevisiae has been described. PMID: 27321754
  2. A dynamic Ifh1-Utp22 interaction fine-tunes ribosomal protein gene (RPG) expression to coordinate RPG and rRNA transcription. PMID: 27818142
  3. data suggest a novel mechanism of regulation whereby Gcn5 functions to titrate the activity of Ifh1 following its recruitment to RP promoters to provide more than an all-or-nothing mode of transcriptional regulation PMID: 23973296
  4. activation domain (AD) of the ribosomal protein genes activator Ifh1p was identified. PMID: 20059977
  5. Ifh1 association with promoters is the key regulatory step for coordinate expression of ribosomal protein genes PMID: 15616568
  6. central role for Ifh1 and Fhl1 in ribosomal protein gene regulation PMID: 15616569
  7. Fhl1p and Ifh1p are key players in the transcription of ribosomal protein genes PMID: 15692568
  8. the interaction between Fhl1p and Ifh1p is critical for the regulation of CPC2 transcription during utilization of different carbon sources. PMID: 16402205

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Subcellular Location
Nucleus.
Protein Families
IFH1 family
Database Links

KEGG: sce:YLR223C

STRING: 4932.YLR223C

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