ligD Antibody

Code CSB-PA620474XA01MVX
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Product Details

Full Product Name
Rabbit anti-Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) ligD Polyclonal antibody
Uniprot No.
Target Names
ligD
Alternative Names
ligD antibody; MSMEG_5570 antibody; MSMEI_5419Multifunctional non-homologous end joining protein LigD antibody; NHEJ DNA polymerase) [Includes: DNA repair polymerase antibody; Pol antibody; Polymerase/primase); 3'-phosphoesterase antibody; 3'-ribonuclease/3'-phosphatase antibody; PE); DNA ligase antibody; Lig antibody; EC 6.5.1.1 antibody; Polydeoxyribonucleotide synthase [ATP])] antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Immunogen
Recombinant Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) ligD protein
Immunogen Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
With Ku forms a non-homologous end joining (NHEJ) repair enzyme which repairs blunt-end and 5'-overhang DNA double strand breaks (DSB) with about 50% fidelity, and DSB with non-complementary 3' ends. Plays a partial role in NHEJ during 3'-overhang repair. NHEJ repairs DSB with blunt ends and 5' overhangs with a high level of nucleotide insertion/deletion, without a need for microhomology. Acts as a DNA ligase on singly nicked dsDNA, as a DNA-directed DNA polymerase on 5' overhangs, and adds non-templated nucleotides to 3' overhangs (terminal transferase). Fills in gaps in dsDNA, prefers a 5'-phosphate in the gap. Site-directed mutations leading to ligase loss alter the bias from insertion to deletion mutations, and indicate another ligase (LigC1 and/or LigC2) can compensate.; The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool may be limiting.; The ligase activity is required for replication of viruses with short cos ends (4 bases) such as Mycobacterium phage Omega and Corndog, but not D29 which has a 9 base cos end. Stimulates dsDNA end joining by LigD; when expressed with endogenous or Mycobacterium phage Omega Ku, can reconstitute NHEJ in S.cerevisiae.
Protein Families
LigD polymerase family; LigD 3'-phosphoesterase family; ATP-dependent DNA ligase family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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