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  • Small Molecule

lolE Antibody

Datasheet
Code CSB-PA303464XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) lolE Polyclonal antibody
Uniprot No.
P75958
Target Names
lolE
Alternative Names
lolE; ycfW; b1118; JW1104; Lipoprotein-releasing system transmembrane protein LolE
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) lolE protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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lolE Proteins

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Target Background

Function
Part of an ATP-dependent transport system LolCDE responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of LolA.
Gene References into Functions
  1. Inhibition of LolCDE leads to significant inhibition of growth and lipoprotein transport. PMID: 27645386
  2. These results indicate that the affinity of LolCDE for apolipoprotein is very low, and therefore, overexpression of LolCDE is required for its release and sorting to the outer membrane. PMID: 21742870
  3. suppressor mutations of the lolE gene that correct the growth defect caused by the LolD mutations were mainly located in the cytoplasmic loop PMID: 16585747
  4. Liganded LolCDE thus reveals molecular events brought about through ATP binding and hydrolysis, LolCDE is the first example of an ABC transporter purified with tightly bound native substrates. PMID: 17038124
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
ABC-4 integral membrane protein family, LolC/E subfamily
Database Links
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