Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MAP65-1 Polyclonal antibody

Uniprot No.

Q9FLP0

Target Names

MAP65-1

Alternative Names

MAP65-1 antibody; At5g55230 antibody; MCO15.18 antibody; 65-kDa microtubule-associated protein 1 antibody; AtMAP65-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MAP65-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Microtubule-associated protein that bundle and stabilize adjacent microtubules (MT) of the cell cortex. Enhances MT nucleation. Can also bind to tubulin dimers and promotes their polymerization. Confers MT resistance to the drug propyzamide and cold conditions. Plays a role in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. Represses metaphase spindle organization and the transition to anaphase in dephosphorylated active form. Promotes the formation of a planar network of antiparallel microtubules. May be involved in stomatal movement modulation by regulating the dynamic and arrangement of cortical MT.

Gene References into Functions

MAP65-1 plays a critical role in the response to salt stress and is required for regulating the rapid depolymerization and reorganization of cortical microtubules (MTs). PMID: 28969791
MAP65-1 is subject to multiple phosphorylation events by Aurora kinases during consecutive stages of the cell cycle. PMID: 27879390
MAP65-1 and MAP65-2 play a critical role in the microtubule-dependent mechanism for specifying axial cell growth in the expanding hypocotyl, independent of any mechanical role in microtubule array organization. PMID: 21551389
Results also show that MAP65-1 and MAP65-3 may share redundant functions in MT stabilization. PMID: 22570443
the rod domain of MAP65-1 acts both as a spacer and as a structural element that specifies the microtubule encounter angles that are conducive for bundling PMID: 22385851
AtMAP65-1 plays a role in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. PMID: 16098102
Data suggest that normal metaphase spindle organisation and the transition to anaphase is dependent on inactivation of AtMAP65-1 PMID: 16847052
AtMAP65-1 might promote tubulin assembly by binding to tubulin dimers in vivo.[AtMAP65-1] PMID: 17394772
The predicted three-dimensional structure of AtMAP65-1 was used as a basis for analyzing the actual cross-bridging with microtubules in detail. PMID: 17504815
MAP65-1 binds cortical microtubules. PMID: 17674780
AtMAP65-1 and AtMAP65-5 promote antiparallel microtubule bundling PMID: 18667529

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cell cortex. Note=During interphase, binds cortical microtubules. In M-phase, locates to the preprophase band. Binds the preprophase band but does not accumulate at the prophase spindle microtubules that coexists within the same cell. In the metaphase and anaphase, localized to spindle. In the phragmoplast, concentrated at the midzone where vesicles guide by MT coalesce to form the cell plate. Present in MT cortical arrays. Concentrated in dots surrounding the nuclei.

Protein Families

MAP65/ASE1 family

Tissue Specificity

Expressed in all organs and tissues with the exception of sepals and anthers. Bound to subsets of microtubules in the cells of root epidermis, hypocotyl and cotyledons (at protein level).

Database Links

KEGG: ath:AT5G55230

STRING: 3702.AT5G55230.2

UniGene: At.7440

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MAP65-1 Antibody

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