MAP65-4 Antibody

Datasheet

Code	CSB-PA878651XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MAP65-4 Polyclonal antibody

Uniprot No.

Q9LZY0

Target Names

MAP65-4

Alternative Names

MAP65-4 antibody; At3g60840 antibody; T4C21.25065-kDa microtubule-associated protein 4 antibody; AtMAP65-4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MAP65-4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Microtubule-associated protein involved in mitotic spindle formation.

Gene References into Functions

MAP65-4 has a role in engaging and bundling anti-parallel MTs in the phragmoplast. PMID: 28370001
Data indicate that microtubules (MTs) bundle flexibility is differently regulated, depending on MAP65 cross-linker. PMID: 23615441
Data show that Arabidopsis thaliana MAP65-4 specifically associates with the forming mitotic spindle during prophase and with the kinetochore fibers from prometaphase to the end of anaphase. PMID: 21119057
Subcellular localization of MAP65-3 and analysis of microtubule organization in the mutant demonstrated that MAP65-3 played a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. PMID: 18263774

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Localized specifically to MT that rearranged to form a spindle and the polar sides of the spindle proper. Associates to MT in preprophase band, during anaphase, and in phragmoplast.

Protein Families

MAP65/ASE1 family

Database Links

KEGG: ath:AT3G60840

STRING: 3702.AT3G60840.1

UniGene: At.54023

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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MAP65-4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background