AMC9 Antibody

Code CSB-PA884349XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AMC9 Polyclonal antibody
Uniprot No.
Target Names
AMC9
Alternative Names
AMC9 antibody; MCP2F antibody; At5g04200 antibody; F21E1.120Metacaspase-9 antibody; AtMC9 antibody; EC 3.4.22.-) [Cleaved into: Metacaspase-9 subunit p20; Metacaspase-9 subunit p10 antibody; Metacaspase 2f antibody; AtMCP2f)] antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AMC9 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Required for proteolytic processing of GRI.
Gene References into Functions
  1. The substrate specificity of MC9 was characterized. PMID: 23964026
  2. The function of AtMC9 in xylem cell death is to degrade vessel cell contents after vacuolar rupture. PMID: 23834670
  3. the mature processed form is not prone to NO inhibition due to the presence of a second S-nitrosylation-insensitive cysteine that can replace the S-nitrosylated cysteine residue within the catalytic center of the processed AtMC9 PMID: 17110382
Subcellular Location
Secreted, extracellular space, apoplast.
Protein Families
Peptidase C14B family
Tissue Specificity
Expressed in root tips, cauline leaves, flowers and siliques.
Database Links

KEGG: ath:AT5G04200

STRING: 3702.AT5G04200.1

UniGene: At.27184

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