Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MET30 Polyclonal antibody

Uniprot No.

P39014

Target Names

MET30

Alternative Names

MET30 antibody; YIL046W antibody; F-box protein MET30 antibody; E3 ubiquitin ligase complex SCF(Met30) subunit MET30 antibody; Methionine-requiring protein 30 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MET30 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Substrate-recognition component of the SCF(Met30) complex, an E3 ubiquitin ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the SCF(Met30) complex. Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Required for efficient expression of G1 cyclins. The SCF(Met30) complex catalyzes ubiquitination and degradation of the Cdk-inhibitory kinase SWE1. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. The SCF(Met30) complex mediates ubiquitination and subsequent degradation of MET4 and the cellular response to cadmium. The SCF(Met30) complex acts as an inhibitor of autophagy by promoting ubiquitination and degradation of ATG9 in normal conditions.

Gene References into Functions

In Saccharomyces cerevisiae, transcription of the MET regulon is controlled by ubiquitination of the transcription factor Met4, which is carried out by the SCF(Met30) E3 ubiquitin ligase. PMID: 24648496
The stability of these DNA-binding cofactors is regulated by SCF(Met30). PMID: 21172660
The association of Met30 with Skp1 but not with its substrate Met4 was inhibited in cells treated with cadmium PMID: 15689486
data suggest that Met30 regulates several aspects of the cell cycle, including G(1)-specific transcription, initiation of DNA replication, and progression through M phase PMID: 15870262
the recruitment of Met4p to the SCF(Met30p) complex itself results in inactivation of Met4p, independently of its ubiquitination PMID: 15883825

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

WD repeat MET30/SCONB/SCON-2 family

Database Links

KEGG: sce:YIL046W

STRING: 4932.YIL046W

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MET30 Antibody

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