HAB1 Antibody

Code CSB-PA861273XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HAB1 Polyclonal antibody
Uniprot No.
Target Names
HAB1
Alternative Names
HAB1 antibody; P2C-HA antibody; At1g72770 antibody; F28P22.4 antibody; Protein phosphatase 2C 16 antibody; AtPP2C16 antibody; EC 3.1.3.16 antibody; AtP2C-HA antibody; Protein HYPERSENSITIVE TO ABA 1 antibody; Protein phosphatase 2C HAB1 antibody; PP2C HAB1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HAB1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Confers enhanced sensitivity to drought.
Gene References into Functions
  1. HAB1.1 and HAB1.2, that play opposing roles in abscisic acid -mediated seed germination and ABA-mediated post-germination. developmental arrest. PMID: 26419884
  2. We have identified HAB1 C186 and C274 as H2O2-sensitive thiols and demonstrate that their oxidation inhibits both HAB1 catalytic activity and its ability to physically associate with SnRK2.6 by formation of intermolecular dimers. PMID: 25460914
  3. Data demonstrated that HAB1 dephosphorylates and deactivates OST1 in vitro. HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. PMID: 19855047
  4. PYL5 binds ABA with micromolar or nanomolar affinity in the absence or presence of HAB1, respectively. PMID: 19624469
  5. show that plants expressing a mutant form of HAB1 in which Gly246 was mutated to Asp (G246D) display strong ABA insensitive phenotypes PMID: 16876791
  6. HAB1 modulates the abscisic acid response by regulation SWI3B. PMID: 19033529
  7. findings show that abscissic binds to PYRABACTIN RESISTANCE 1 (PYR1), which in turn binds to and inhibits Type 2C protein phosphatases (HAB1) PMID: 19407142

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Subcellular Location
Cytoplasm. Nucleus. Note=Mainly cytoplasmic.
Protein Families
PP2C family
Tissue Specificity
Expressed in seeds, roots, stems, leaves and flowers, especially in meristematic tissues, guard cells, embryo and siliques.
Database Links

KEGG: ath:AT1G72770

STRING: 3702.AT1G72770.1

UniGene: At.46635

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