PEX4 Antibody

Code CSB-PA836558XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PEX4 Polyclonal antibody
Uniprot No.
Target Names
PEX4
Alternative Names
PEX4 antibody; UBC21 antibody; At5g25760 antibody; F18A17.10 antibody; Protein PEROXIN-4 antibody; AtPEX4 antibody; E2 ubiquitin-conjugating enzyme 21 antibody; Probable ubiquitin-conjugating enzyme E2 21 antibody; EC 2.3.2.23 antibody; Ubiquitin carrier protein 21 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PEX4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
Gene References into Functions
  1. Growth at elevated temperature did not reduce the fraction of membrane-associated PEX5 in pex4-1 mutants, suggesting that elevated temperature did not restore PEX4 enzymatic function in the mutant. PMID: 26377801
  2. PEX4 is necessary for Arabidopsis peroxisomal function. pex4 mutants are sucrose dependent during germination and display reduced responses to the auxin precursor indole-3-butyric acid, presumably due to peroxisomal beta-oxidation defects. PMID: 16272432
Subcellular Location
Peroxisome membrane; Peripheral membrane protein.
Protein Families
Ubiquitin-conjugating enzyme family
Database Links

KEGG: ath:AT5G25760

STRING: 3702.AT5G25760.1

UniGene: At.20548

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