PIE1 Antibody

Code CSB-PA187810XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIE1 Polyclonal antibody
Uniprot No.
Target Names
PIE1
Alternative Names
PIE1 antibody; CHR13 antibody; At3g12810 antibody; MBK21.19 antibody; Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 antibody; EC 3.6.4.12 antibody; Independent early flowering 1 protein antibody; Protein CHROMATIN REMODELING 13 antibody; AtCHR13 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIE1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Probable DNA-dependent ATPase. Not involved in the repression of FLC in gametophytes, but required for the reactivation of FLC in early embryos and for the maintenance of full activation of FLC in late embryos.
Gene References into Functions
  1. Data indicate that mutations in genes for SWR1 complex subunits photoperiod-independent Early Flowering1 (PIE1), actin-related protein6 (ARP6), and SWR1 complex6 (SWC6) cause hypersensitivity to various DNA damaging agents. PMID: 23780875
  2. The identification and characterization of SERRATED LEAVES AND EARLY FLOWERING (SEF) in A. thaliana and its interactions with other proteins, including ATARP6 and PIE1, are reported. PMID: 17142478
  3. Arabidopsis SWC6 (AtSWC6), SUPPRESSOR OF FRIGIDA 3 (SUF3) and PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (PIE1) are homologs of SWC6, ARP6 and SWR1 with roles in development, including floral repression through full activation of FLOWERING LOCUS C PMID: 17470967
Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family, SWR1 subfamily
Tissue Specificity
Expressed in ovules, but not in stamens.
Database Links

KEGG: ath:AT3G12810

STRING: 3702.AT3G12810.1

UniGene: At.39549

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