PRORP2 Antibody

Code CSB-PA193713XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PRORP2 Polyclonal antibody
Uniprot No.
Target Names
PRORP2
Alternative Names
PRORP2 antibody; At2g16650 antibody; T24I21.6 antibody; Proteinaceous RNase P 2 antibody; EC 3.1.26.5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PRORP2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially binds precursor tRNAs containing short 5' leaders and 3' trailers. Also involved in the maturation of mRNA and small nucleolar RNA (snoRNA).
Gene References into Functions
  1. The comparative biology of Escherichia coli RNase P and Arabidopsis thaliana PRORP isozymes has been reported. PMID: 28499021
  2. Data suggest that PRORP2 undergoes conformational changes to accommodate its substrate (precursor tRNA) and product (mature tRNA); the catalytic domain and the RNA-binding domain appear to move around a central hinge; cysteine-specific tRNA(GCA) was used in these experiments. PMID: 28696260
  3. The purification and crystallization of PRORP2 from Arabidopsis was identified. PMID: 26527263
  4. The authors solved the crystal structure of PRORP2 (3.2A) revealing an overall V-shaped protein and conserved metallonuclease active-site structure. PMID: 26655022
  5. The study presents the first kinetic analysis of PRORP1, PRORP2 and PRORP3. PMID: 22976545

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Subcellular Location
Nucleus.
Protein Families
PPR family, P subfamily
Database Links

KEGG: ath:AT2G16650

STRING: 3702.AT2G16650.1

UniGene: At.29161

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