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purB Antibody

Datasheet
Code CSB-PA001397XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) purB Polyclonal antibody
Uniprot No.
P0AB89
Target Names
purB
Alternative Names
purB antibody; b1131 antibody; JW1117 antibody; Adenylosuccinate lyase antibody; ASL antibody; EC 4.3.2.2 antibody; Adenylosuccinase antibody; ASase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) purB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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purB Proteins

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Target Background

Function
Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.; (Microbial infection) Catalyzes the conversion of 2-amino-2'-deoxyadenylo-succinate to dZMP and fumarate, when the bacterium is infected by a phage that produces the substrate of this reaction, a step in the synthesis of dZTP (2-amino-2'-deoxyadenosine 5'-triphosphate), which is a nucleotide then used by the phage as a DNA polymerase substrate.
Gene References into Functions
  1. crystal structures of wild-type Escherichia coli ADL (ec-ADL), and mutant-substrate (H171A-ADS) and -product (H171N-AMP.FUM) complexes to 2.0, 1.85, and 2.0 A resolution, respectively PMID: 17531264
Protein Families
Lyase 1 family, Adenylosuccinate lyase subfamily
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