PYM Antibody

Datasheet

Code	CSB-PA571325XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PYM Polyclonal antibody

Uniprot No.

O48533

Target Names

PYM

Alternative Names

PYM antibody; UVI4 antibody; At2g42260 antibody; T24P15.17 antibody; Protein POLYCHOME antibody; Protein UV-B-INSENSITIVE 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PYM protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Negative regulator of the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase required for proper mitotic progression and cell fate determination; inhibits premature cell differentiation. Prevents DNA endoreplication by promoting the maintenance of the mitotic state by preferentially inhibiting APC/C(FZR) and triggering cyclins accumulation (e.g. CYCB1-1, CYCB1-2 and CYCA2-3) in a temporal manner. Required for megagametophyte and endosperm development. Counteracts the activity of CCS52A1 thus inhibiting the turnover of CYCA2-3. Confers immunity to bacterial pathogens (e.g. Pseudomonas syringae pv. tomato DC3000), which is associated with increased expression of disease resistance (R) genes.

Gene References into Functions

UBIQUITIN-SPECIFIC PROTEASE14 Interacts with ULTRAVIOLET-B INSENSITIVE4 to Regulate Endoreduplication and Cell and Organ Growth in Arabidopsis PMID: 27099260
CPR5 has a large role in cell cycle regulation and this role has a complex interaction with that of UVI4 and OSD1. PMID: 24945150
These findings suggest that endomitosis and endoreplication are regulated by similar molecular mechanisms, in which two related proteins, GIG1 and UVI4, may inhibit APC/C in different ways. PMID: 22899078
GIGAS CELL1 (GIG1)/OMISSION OF SECOND DIVISION1 (OSD1) and UVI4 encode novel plant-specific inhibitors of the anaphase-promoting complex/cyclosome ubiquitin ligase. PMID: 22167058
UVI4 inhibits premature cell differentiation. PMID: 22167059

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Subcellular Location

Nucleus. Note=Follows a patchy nuclear accumulation pattern.

Tissue Specificity

Expressed mainly in actively dividing cells (e.g. central cylinder of the root tip, young leaves and vascular tissues).

Database Links

KEGG: ath:AT2G42260

STRING: 3702.AT2G42260.1

UniGene: At.42734

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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