RAP2-12 Antibody

Datasheet

Code	CSB-PA882943XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAP2-12 Polyclonal antibody

Uniprot No.

Q9SSA8

Target Names

RAP2-12

Alternative Names

RAP2-12 antibody; ERF074 antibody; At1g53910 antibody; T18A20.14 antibody; Ethylene-responsive transcription factor RAP2-12 antibody; Protein RELATED TO APETALA2 12 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RAP2-12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Transcription factor involved in the activation of hypoxic gene expression and in ethylene response. Partially redundant with RAP2-2. Acts as a downstream regulator in the ethylene signaling pathway.

Gene References into Functions

ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF74] PMID: 28164334
Hypoxic root bending is inhibited by the group VII ethylene response factor (ERFVII) RAP2.12, as rap2.12-1 seedlings show exaggerated primary root bending. PMID: 28698356
Results show that oxygen-sensing, mediating transcription factor RAP2.12 degradation is indispensable to optimize metabolic performance, plant growth, and development under both normoxic and hypoxic conditions. PMID: 27372243
In Arabidopsis, an effective low oxygen-sensing response requires RAP2.12 stabilization followed by HRA1 induction to modulate the extent of the anaerobic response by negative feedback regulation of RAP2.12. PMID: 25226037
identification of a conserved amino-terminal amino acid sequence of RAP2.12 to be dedicated to an oxygen-dependent sequence of post-translational modifications, which ultimately lead to degradation of RAP2.12 under aerobic conditions PMID: 22020282

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Subcellular Location

Cell membrane. Nucleus. Note=Localizes to the plasma membrane under aerobic conditions, but accumulates in the nucleus upon hypoxia.

Protein Families

AP2/ERF transcription factor family, ERF subfamily

Tissue Specificity

Highly expressed in seedlings, leaves, flowers, siliques and germinating seeds.

Database Links

KEGG: ath:AT1G53910

STRING: 3702.AT1G53910.1

UniGene: At.20952

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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RAP2-12 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background