RBOHF Antibody

Code CSB-PA528628XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RBOHF Polyclonal antibody
Uniprot No.
Target Names
RBOHF
Alternative Names
RBOHF antibody; RBOHAP108 antibody; At1g64060 antibody; F22C12.18 antibody; Respiratory burst oxidase homolog protein F antibody; EC 1.11.1.- antibody; EC 1.6.3.- antibody; Cytochrome b245 beta chain homolog RbohAp108 antibody; NADPH oxidase RBOHF antibody; AtRBOHF antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RBOHF protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Calcium-dependent NADPH oxidase that generates superoxide. Generates reactive oxygen species (ROS) during incompatible interactions with pathogens and is important in the regulation of the hypersensitive response (HR). Involved in abscisic acid-induced stomatal closing and in UV-B and abscisic acid ROS-dependent signaling.
Gene References into Functions
  1. RbohF is responsible for the RPK1-triggered superoxide production. PMID: 29262318
  2. this study revealed that the AtrbohF-dependent ROS signaling is required for melatonin-induced salinity tolerance via the reestablishment of ion and redox homeostasis PMID: 28412199
  3. Ethylene promotes soil-salinity tolerance via improved Na/K homeostasis mediated by RBOHF-dependent regulation of Na accumulation and RBOHF-independent regulation of K accumulation. PMID: 24064768
  4. AtRbohF appears to be a key player not only in HR-related cell death but also in regulating metabolomic responses and resistance. PMID: 21985584
  5. These results suggest that AtRbohF is probably involved in post-penetration resistance to Magnaporthe oryzae in Arabidopsis. PMID: 23748772
  6. Phosphatidic acid bound to recombinant Arabidopsis NADPH oxidase RbohD (respiratory burst oxidase homolog D) and RbohF. PMID: 19690149

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
RBOH (TC 5.B.1.3) family
Tissue Specificity
Expressed in roots, stems, seedlings, inflorescences, leaves and guard cells.
Database Links

KEGG: ath:AT1G64060

STRING: 3702.AT1G64060.1

UniGene: At.235

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