RAD52-1 Antibody

Code CSB-PA136452XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAD52-1 Polyclonal antibody
Uniprot No.
Target Names
RAD52-1
Alternative Names
RAD52-1 antibody; ODB1 antibody; At1g71310 antibody; F3I17.4 antibody; DNA repair RAD52-like protein 1 antibody; mitochondrial antibody; Organellar DNA-binding protein 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAD52-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plant-specific single-stranded DNA-binding protein required for efficient heterologous recombination-dependent DNA repair in nuclear and mitochondrial compartments. Forms large nucleo-protein complexes with WHY2 in mitochondria. Binds ssDNA with high affinity, but with little sequence specificity. Involved in double-stranded DNA break repair. Involved in the hydrolytic splicing pathway in mitochondrion. Facilitates the excision of two cis-spliced group II introns, NAD1 intron 2 and NAD2 intron 1.
Gene References into Functions
  1. The results demonstrate a role for the protein ODB1 in group-II intron splicing in mitochondria of Arabidopsis. PMID: 26048959
  2. Arabidopsis odb1 mutants were morphologically indistinguishable from the wild-type, but following DNA damage by genotoxic stress, they showed reduced mitochondrial homologous recombination activity. PMID: 22762281
Subcellular Location
Mitochondrion. Nucleus.
Protein Families
RAD52 family
Tissue Specificity
Expressed in root vascular tissue, tips of primary and secondary roots, young leaves, hydathodes, stomatal guard cells, cauline leaves, flower buds, stipules, carpels, pistils and anther filaments.
Database Links

KEGG: ath:AT1G71310

STRING: 3702.AT1G71310.1

UniGene: At.70183

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